Linkage of oxidative stress and mitochondrial dysfunctions to spontaneous culture degeneration in Aspergillus nidulans.

Molecular & Cellular Proteomics : MCP
Lin LiChengshu Wang

Abstract

Filamentous fungi including mushrooms frequently and spontaneously degenerate during subsequent culture maintenance on artificial media, which shows the loss or reduction abilities of asexual sporulation, sexuality, fruiting, and production of secondary metabolites, thus leading to economic losses during mass production. To better understand the underlying mechanisms of fungal degeneration, the model fungus Aspergillus nidulans was employed in this study for comprehensive analyses. First, linkage of oxidative stress to culture degeneration was evident in A. nidulans. Taken together with the verifications of cell biology and biochemical data, a comparative mitochondrial proteome analysis revealed that, unlike the healthy wild type, a spontaneous fluffy sector culture of A. nidulans demonstrated the characteristics of mitochondrial dysfunctions. Relative to the wild type, the features of cytochrome c release, calcium overload and up-regulation of apoptosis inducing factors evident in sector mitochondria suggested a linkage of fungal degeneration to cell apoptosis. However, the sector culture could still be maintained for generations without the signs of growth arrest. Up-regulation of the heat shock protein chaperones, anti-apopt...Continue Reading

References

Jan 5, 2000·Cell Motility and the Cytoskeleton·R Suelmann, R Fischer
Apr 25, 2000·Molecular and Cellular Biology·C VoisineE A Craig
Jan 26, 2002·Molecular Biology of the Cell·Patrick J Westfall, Michelle Momany
Jun 12, 2002·The Journal of Cell Biology·Stephen R CroninRandolph Y Hampton
Jun 18, 2002·Journal of Applied Microbiology·M J RyanP Jeffries
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Ana M CalvoNancy P Keller
Sep 27, 2002·Experimental Biology and Medicine·Yau-Huei Wei, Hsin-Chen Lee
May 13, 2003·Cell Motility and the Cytoskeleton·Katrin V KochReinhard Fischer
May 27, 2003·Apoptosis : an International Journal on Programmed Cell Death·J D LyA Lawen
Jul 18, 2003·Cell Death and Differentiation·M Karbowski, R J Youle
Aug 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ji-Ming GongJulian I Schroeder
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Apr 28, 2004·Journal of Invertebrate Pathology·Chengshu WangTariq M Butt
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Paul S BrookesShey-Shing Sheu
Sep 24, 2004·The Journal of Cell Biology·Silke WissingFrank Madeo
Nov 24, 2004·Molecular Microbiology·Lori A Maggio-Hall, Nancy P Keller
Feb 3, 2005·Free Radical Biology & Medicine·Daniela A BotaKelvin J A Davies
Mar 1, 2005·Cell·Robert S BalabanToren Finkel
Jul 5, 2005·Current Opinion in Microbiology·Charles E Bullerwell, B Franz Lang
Sep 20, 2005·FEMS Microbiology Letters·Farooq A ShahTariq M Butt
Oct 7, 2005·Microbiology·Chengshu WangRaymond J St Leger
Jul 1, 1994·Applied and Environmental Microbiology·A LiP A Horgen
Mar 31, 2007·Biochemical and Biophysical Research Communications·Hanyu LiangQitao Ran
Apr 5, 2007·Nature Protocols·Edyta SzewczykBerl Oakley
Apr 5, 2008·Biotechnology Progress·Bill J MossMark R Marten
Jun 19, 2008·Genes & Development·Der-Fen SuenRichard J Youle
Sep 10, 2008·Molecular Biotechnology·Matthias ElstnerHolger Prokisch
May 5, 2009·Biochimica Et Biophysica Acta·Uwe SchlattnerRichard M Epand
Jun 30, 2009·Trends in Biochemical Sciences·Ian J Holt
Jul 7, 2009·Biochimica Et Biophysica Acta·John J LemastersAnna-Liisa Nieminen
Jul 25, 2009·Journal of Proteome Research·Nitin Gupta, Pavel A Pevzner
Dec 17, 2009·Nature Biotechnology·Noelle M GriffinJan E Schnitzer
Mar 17, 2010·Trends in Plant Science·Olivier Van AkenFrank Van Breusegem
Jun 12, 2010·Annals of the New York Academy of Sciences·Heinz D OsiewaczIngmar Strobel

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Citations

Feb 6, 2016·Scientific Reports·Éva LeiterIstván Pócsi
Dec 3, 2014·Fungal Genetics and Biology : FG & B·Yixiong ChenChengshu Wang
Jun 28, 2014·Fungal Genetics and Biology : FG & B·Katerina GalanopoulouSotiris Amillis
Jun 6, 2018·Microorganisms·Vera Meyer, Sascha Jung
Oct 11, 2017·Antimicrobial Agents and Chemotherapy·Emina JukicMichaela Lackner
Dec 15, 2019·Applied and Environmental Microbiology·Xiaoxiang YangYanping Fu
Feb 24, 2017·World Journal of Microbiology & Biotechnology·Facundo Muñiz-ParedesOctavio Loera
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Wei HuangChengshu Wang
Jun 28, 2019·Fungal Genetics and Biology : FG & B·Roya Vahedi Shahandashti, Cornelia Lass-Flörl

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