DOI: 10.1101/484022Nov 30, 2018Paper

Linked-read sequencing of gametes allows efficient genome-wide analysis of meiotic recombination

BioRxiv : the Preprint Server for Biology
Hequan SunKorbinian Schneeberger

Abstract

Meiotic crossovers (COs) ensure proper chromosome segregation and redistribute the genetic variation that is transmitted to the next generation. Existing methods for CO identification are challenged by large populations and the demand for genome-wide and fine-scale resolution. Taking advantage of linked-read sequencing, we developed a highly efficient method for genome-wide identification of COs at kilobase resolution in pooled recombinants. We first tested this method using a pool of Arabidopsis F2 recombinants, and obtained results that recapitulated those identified from the same plants using individual whole-genome sequencing. By applying this method to a pool of pollen DNA from a single F1 plant, we established a highly accurate CO landscape without generating or sequencing a single recombinant plant. The simplicity of this approach now enables the simultaneous generation and analysis of multiple CO landscapes and thereby allows for efficient comparison of genotypic and environmental effects on recombination, accelerating the pace at which the mechanisms for the regulation of recombination can be elucidated.

Related Concepts

Chromosomes
DNA
Environment
Genome
Germ Cells
Recombinant Proteins
Recombination, Genetic
Arabidopsis thaliana <plant>
Landscapes
Analysis

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