PMID: 8612630Mar 1, 1996Paper

Linking of isozyme and class variability patterns in the emergence of novel alcohol dehydrogenase functions. Characterization of isozymes in Uromastix hardwickii

European Journal of Biochemistry
L HjelmqvistH Jörnvall

Abstract

The nature of the isozyme differences in the class-I alcohol dehydrogenase structure from the lizard, Uromastix hardwickii, was determined and related to those in the human and horse enzymes, for which isozyme structures have also been established. The Uromastix isozymes differ much (at a total of 72 positions, 19%) but, in spite of this, have similar properties and were not obtained resolved. Their structures were analyzed in mixture, and the two sub-sets of peptides obtained could be distinguished by evaluation of the recovery ratios within the peptide pairs. The isozymes have class-I activities, with an ethanol dehydrogenase activity of 0.6 U/mg and no formaldehyde dehydrogenase activity, have typical class-I structures, and are composed of N-terminally acetylated 375-residue subunits (a and b). Importantly, variability patterns between the isozymes are reminiscent of those both in the other two lines with isozymes (primates and horse) and in the class distinctions of the enzyme. Hence, the variability pattern since the distant stage of class-I emergence is also visible within the more recent isozyme divergence, illustrating a continuity in the evolution of isozymes to classes (and then to enzymes). The pattern also links th...Continue Reading

References

Jan 1, 1992·Protein Science : a Publication of the Protein Society·D C Richardson, J S Richardson
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·O Danielsson, H Jörnvall
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T D HurleyL M Amzel
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Sep 1, 1970·European Journal of Biochemistry·H Jörnvall
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·L HjelmqvistH Jörnvall
May 9, 1995·Biochemistry·H JörnvallD Ghosh
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·O DanielssonH Jörnvall

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Citations

Jul 29, 1999·Biochemical Pharmacology·G DuesterH Jörnvall
Dec 16, 1998·The Journal of Biological Chemistry·T LuqueR González-Duarte

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