Linking proteins with anionic nanoparticles via protamine: ultrasmall protein-coupled probes for magnetic resonance imaging of apoptosis

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Eyk SchellenbergerBernd Hamm

Abstract

Magnetic resonance imaging (MRI) of a target in vivo depends on the surface, size, and particle relaxivity of the target-specific nanoparticles for MRI. Here a new method for decorating very small iron oxide particles (VSOPs) with target-specific ligands is described. The method is based on the electrostatic attraction of the strongly positively charged peptide protamine to the anionic citrate shell of the electrostatically stabilized VSOPs. The protamine coat allows linkage chemistry and chimera technology to functionalize VSOPs or other negative charged surfaces with biologics. Annexin A5 (anxA5)-VSOP utilizing thiol chemistry was generated to couple biologically active anxA5 to VSOPs for in vivo MRI of apoptosis. Annexin A5-VSOP comprises five anxA5 molecules per iron oxide nanoparticle with a high R2 particle relaxivity of 180 000 mM(-1) s(-1) yet small hydrodynamic diameter of only 14.7+/-2.9 nm beneficial for in vivo MRI of extravascular targets.

References

Nov 15, 1992·European Journal of Biochemistry·A Lewit-BentleyG Bodo
Nov 1, 1981·Cell·S AltmanN A Wosnick
May 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·T ShenT J Brady
Jul 19, 2003·Neoplasia : an International Journal for Oncology Research·Eyk A SchellenbergerLee Josephson
Sep 16, 2004·Bioconjugate Chemistry·Eyk A SchellenbergerLee Josephson
Aug 9, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·David E SosnovikLee Josephson
Nov 23, 2006·Advanced Drug Delivery Reviews·Claire CorotMarc Port
May 17, 2007·Anti-cancer Agents in Medicinal Chemistry·Gustav J StrijkersKlaas Nicolay
Jun 20, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Marina KartachovaRenato Alfredo Valdés Olmos

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Citations

Aug 13, 2008·Der Radiologe·T PersigehlC Bremer
May 5, 2010·Apoptosis : an International Journal on Programmed Cell Death·Kristof Schutters, Chris Reutelingsperger
Sep 2, 2010·Bioconjugate Chemistry·Geralda A F van TilborgKlaas Nicolay
Jul 1, 2011·Nanoscale·Morteza MahmoudiSophie Laurent
Nov 1, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ting-Jung ChenYun-Ming Wang
May 23, 2012·Colloids and Surfaces. B, Biointerfaces·Myung-Ik YooKyoungja Woo
Apr 16, 2011·Nuclear Medicine and Biology·Matthias BauwensAlfons Verbruggen
Nov 17, 2009·Advanced Drug Delivery Reviews·Omid VeisehMiqin Zhang
Jan 13, 2009·Contrast Media & Molecular Imaging·Geralda A F van TilborgKlaas Nicolay
Oct 15, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Howard H ChenDavid E Sosnovik
Apr 11, 2012·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Veronica Clavijo-JordanKevin M Bennett
Apr 8, 2014·Contrast Media & Molecular Imaging·Lena FiggeEyk Schellenberger
Jul 24, 2014·Korean Journal of Radiology : Official Journal of the Korean Radiological Society·Mijung JangSun Mi Kim
Feb 2, 2009·Advanced Materials·Benjamin ThierryHans J Griesser
Oct 9, 2019·Aging Clinical and Experimental Research·Anna TampieriSimone Sprio
Nov 15, 2008·Bioconjugate Chemistry·Eyk SchellenbergerJörg Schnorr

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis