Linocin and OmpW Are Involved in Attachment of the Cystic Fibrosis-Associated Pathogen Burkholderia cepacia Complex to Lung Epithelial Cells and Protect Mice against Infection

Infection and Immunity
Siobhán McCleanMinu Shinoy

Abstract

Members of the Burkholderia cepacia complex (Bcc) cause chronic opportunistic lung infections in people with cystic fibrosis (CF), resulting in a gradual lung function decline and, ultimately, patient death. The Bcc is a complex of 20 species and is rarely eradicated once a patient is colonized; therefore, vaccination may represent a better therapeutic option. We developed a new proteomics approach to identify bacterial proteins that are involved in the attachment of Bcc bacteria to lung epithelial cells. Fourteen proteins were reproducibly identified by two-dimensional gel electrophoresis from four Bcc strains representative of two Bcc species: Burkholderia cenocepacia, the most virulent, and B. multivorans, the most frequently acquired. Seven proteins were identified in both species, but only two were common to all four strains, linocin and OmpW. Both proteins were selected based on previously reported data on these proteins in other species. Escherichia coli strains expressing recombinant linocin and OmpW showed enhanced attachment (4.2- and 3.9-fold) to lung cells compared to the control, confirming that both proteins are involved in host cell attachment. Immunoproteomic analysis using serum from Bcc-colonized CF patients c...Continue Reading

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Citations

Apr 20, 2016·Vaccines·Gonzalo A PradenasAlfredo G Torres
Dec 6, 2017·Antimicrobial Resistance and Infection Control·Omar M El-HalfawyMiguel A Valvano
Jun 4, 2020·MSystems·Daniel MännleLeonard Kaysser
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Dec 17, 2020·Cells·Guanbo WangMiguel A Valvano
Aug 7, 2021·Antibiotics·Viola Camilla ScoffoneSilvia Buroni

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