Linoleic Acid Metabolite DiHOME Decreases Post-ischemic Cardiac Recovery in Murine Hearts

Cardiovascular Toxicology
Marwin BannehrAlexander Wutzler

Abstract

Cardiac ischemia/reperfusion injury is associated with the formation and action of lipid mediators derived from polyunsaturated fatty acids. Among them, linoleic acid (LA) is metabolized to epoxyoctadecanoic acids (EpOMEs) by cytochrome P450 (CYP) epoxygenases and further to dihydroxyoctadecanoic acids (DiHOMEs) by soluble epoxide hydrolase (sEH). We hypothesized that EpOMEs and/or DiHOMEs may affect cardiac post-ischemic recovery and addressed this question using isolated murine hearts in a Langendorff system. Hearts from C57Bl6 mice were exposed to 12,13-EpOME, 12,13-DiHOME, or vehicle (phosphate buffered sodium; PBS). Effects on basal cardiac function and functional recovery during reperfusion following 20 min of ischemia were investigated. Electrocardiogram (ECG), left ventricular (LV) pressure and coronary flow (CF) were continuously measured. Ischemia reperfusion experiments were repeated after administration of the sEH-inhibitor 12-(3-adamantan-1-yl-ureido)dodecanoic acid (AUDA). At a concentration of 100 nM, both EpOME and DiHOME decreased post-ischemic functional recovery in murine hearts. There was no effect on basal cardiac parameters. The detrimental effects seen with EpOME, but not DiHOME, were averted by sEH inhib...Continue Reading

References

Jan 1, 1990·Life Sciences·M R SiegfriedR E Zipkin
Feb 13, 1986·Biochemical and Biophysical Research Communications·T OzawaT Tomita
Oct 26, 1994·Molecular and Cellular Biochemistry·K KosakaT Ozawa
Jul 30, 1996·Chemistry and Physics of Lipids·A DuddaF Kobelt
May 1, 1997·Nature Medicine·M F MoghaddamB D Hammock
Jun 15, 1999·Biochimica Et Biophysica Acta·J R StimersD F Grant
Apr 25, 2000·Archives of Biochemistry and Biophysics·J F GreeneB D Hammock
Jul 27, 2001·Archives of Biochemistry and Biophysics·M F SisemoreB D Hammock
Dec 18, 2001·Cardiovascular Research·Nikolaos G FrangogiannisMark L Entman
Sep 18, 2002·The Journal of Pharmacology and Experimental Therapeutics·Maddison D Harrell, Joseph R Stimers
Nov 26, 2003·Progress in Lipid Research·Arthur A SpectorNeal L Weintraub
Dec 20, 2003·Journal of the American College of Nutrition·Saraswathi ViswanathanBernhard Hennig
Jul 22, 2006·Circulation Research·John M SeubertDarryl C Zeldin
Apr 17, 2007·Journal of Biosciences·David Alan Thompson, Bruce D Hammock
May 23, 2007·Heart Failure Reviews·Fabio Di LisaPaolo Bernardi
Oct 7, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Atsuko MotokiDonna M Van Winkle
Oct 2, 2009·Nature Reviews. Drug Discovery·John D Imig, Bruce D Hammock
Sep 28, 2010·Biochimica Et Biophysica Acta·Anne Konkel, Wolf-Hagen Schunck
Jun 24, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Matthew L EdinDarryl C Zeldin
May 23, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Jan Philipp SchuchardtNils Helge Schebb
May 25, 2013·Gene·Todd R Harris, Bruce D Hammock
Jul 19, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Marisol CabralJuan José Moreno
Aug 6, 2014·Biochimica Et Biophysica Acta·Arthur A Spector, Hee-Yong Kim

❮ Previous
Next ❯

Citations

Jul 16, 2019·Physiological Reports·Benjamin GollaschFriedrich C Luft
Jun 21, 2020·Physiological Reports·Benjamin GollaschFriedrich C Luft
Jan 12, 2021·Circulation·Ileana Badi, Charalambos Antoniades
Oct 28, 2020·Circulation·Kelsey M PinckardKristin I Stanford
Feb 12, 2021·International Journal of Molecular Sciences·K Lockhart JamiesonJohn M Seubert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.