Lipase-coated K2SO4 micro-crystals: preparation, characterization, and application in biodiesel production using various oil feedstocks

Bioresource Technology
Jingjing ZhengYunjun Yan

Abstract

This study investigated the preparation and characteristics of protein-coated microcrystals (PCMCs) from Pseudomonas cepacia lipase (PS) and K(2)SO(4), and their application in biodiesel synthesis, via single factorial experiments and response surface methodology (RSM), the optimized PCMC-PS exhibited high activity and stability; the optimal temperature was 60°C (which gave 99.83% conversion), although fairly high activity was exhibited after incubation at different temperatures (25-70°C). The organic solvents stability of the PCMC-PS was improved, and it significantly reduced ethanol toxicity. Circular dichroism (CD) analysis revealed the correlation between the conformation and the enzyme activity. The morphology of the PCMC-PS was also confirmed via scanning electron microscopy (SEM). When catalyzed by PCMC-PS, above 83% biodiesel yield was obtained for most of the seven oils tested. The PCMC-PS (washed with hexane) activity remained relatively stable after eight batch reactions, with only a 15.73% reduction in the conversion (from 99.02% to 83.29%).

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Citations

Jan 5, 2014·Bioresource Technology·Ricca Rahman NasaruddinMohammed Saedi Jami
Feb 14, 2013·Chemical Society Reviews·Patrick Adlercreutz
Nov 28, 2019·World Journal of Microbiology & Biotechnology·Takuya MatsumotoHiroyasu Ogino

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