Lipid Accumulation in Peripheral Blood Dendritic Cells and Anticancer Immunity in Patients with Lung Cancer

Journal of Immunology Research
Ryo AraiYoshiki Ishii

Abstract

We studied the subsets of peripheral blood dendritic cells (DCs) and lipid accumulation in DCs to investigate the involvement of DCs in the decreased anticancer immunity of advanced lung cancer patients. We analyzed the population of DC subsets in peripheral blood using flow cytometry. We then determined lipid accumulation in the DCs using BODIPY 650/665, a fluorophore with an affinity for lipids. Compared with healthy controls, the number of DCs in the peripheral blood of treatment-naive cancer patients was significantly reduced. In patients with stage III + IV disease, the numbers of myeloid DCs (mDCs) and plasmacytoid DCs were also significantly reduced. Lipid accumulation in DCs evaluated based on the fluorescence intensity of BODIPY 650/665 was significantly higher in stage III + IV lung cancer patients than in the controls. In the subset analysis, the fluorescence was highest for mDCs. The intracellularly accumulated lipids were identified as triglycerides. A decreased mixed leukocyte reaction was observed in the mDCs from lung cancer patients compared with those from controls. Taken together, the results show that lung cancer patients have a notably decreased number of peripheral blood DCs and their function as antigen-p...Continue Reading

References

Mar 27, 2002·Nature Reviews. Immunology·Ken Shortman, Yong-Jun Liu
Feb 5, 2003·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D P PenneyC Churukian
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shuwen XuBrian J Czerniecki
Dec 2, 2004·Nature Reviews. Immunology·Dmitry Gabrilovich
Mar 10, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Aki IshikawaTakehiko Fujisawa
Apr 9, 2005·International Journal of Hematology·Tomoki ItoNorimitsu Kadowaki
Jul 14, 2005·Immunology·Asher MaroofStella C Knight
Oct 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christine E LoscherHelen M Roche
Oct 13, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Deirdre O'MahonyMartin E Gutierrez
Feb 7, 2006·Current Gene Therapy·Antoni Ribas
Feb 10, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Scott J AntoniaDmitry I Gabrilovich
Apr 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Manabu ItoJun-ichi Ogawa
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ivan PerrotSerge Lebecque
Sep 1, 2007·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Peter GoldstrawUNKNOWN Participating Institutions
Dec 17, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ofer FainaruDonald E Ingber
Jul 14, 2010·Nature Medicine·Donna L HerberDmitry I Gabrilovich
Sep 21, 2010·European Journal of Immunology·Gerold Schuler
May 10, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·James KrempskiKeith L Knutson
Oct 23, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Daniel S ChenF Stephen Hodi
Jun 2, 2015·The New England Journal of Medicine·Julie BrahmerDavid R Spigel
Sep 29, 2015·The New England Journal of Medicine·Hossein BorghaeiJulie R Brahmer
Oct 11, 2016·The New England Journal of Medicine·Martin ReckUNKNOWN KEYNOTE-024 Investigators

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Citations

Nov 23, 2019·Molecular Omics·J MolendijkM M Hill
Oct 18, 2019·Frontiers in Immunology·Shweta TiwaryMasaki Terabe
Aug 25, 2020·Science Advances·Melissa G MetcalfAndrew Dillin
Mar 19, 2021·Frontiers in Immunology·Xin PengShuang Liu
Sep 5, 2021·The FEBS Journal·Wenfeng ZengWei Liang

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Methods Mentioned

BETA
flow cytometry
fluorescence microscopy
Assay
ELISA
Fluorescence

Software Mentioned

JMP Pro Cary

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