Lipid accumulation, lipid body formation, and acyl coenzyme A oxidases of the yeast Yarrowia lipolytica

Applied and Environmental Microbiology
Katerina MlíckováJean-Marc Nicaud

Abstract

Yarrowia lipolytica contains five acyl-coenzyme A oxidases (Aox), encoded by the POX1 to POX5 genes, that catalyze the limiting step of peroxisomal beta-oxidation. In this study, we analyzed morphological changes of Y. lipolytica growing in an oleic acid medium and the effect of POX deletions on lipid accumulation. Protrusions involved in the uptake of lipid droplets (LDs) from the medium were seen in electron micrographs of the surfaces of wild-type cells grown on oleic acid. The number of protrusions and surface-bound LDs increased during growth, but the sizes of the LDs decreased. The sizes of intracellular lipid bodies (LBs) and their composition depended on the POX genotype. Only a few, small, intracellular LBs were observed in the mutant expressing only Aox4p (Deltapox2 Deltapox3 Deltapox5), but strains expressing either Aox3p or both Aox3p and Aox4p had the same number of LBs as did the wild type. In contrast, strains expressing either Aox2p or both Aox2p and Aox4p formed fewer, but larger, LBs than did the wild type. The size of the LBs increased proportionately with the amount of triacylglycerols in the LBs of the mutants. In summary, Aox2p expression regulates the size of cellular triacylglycerol pools and the size an...Continue Reading

References

Dec 1, 1985·Journal of Steroid Biochemistry·C L BisgaierG V Vahouny
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D A Freeman, M Ascoli
Oct 1, 1984·Applied and Environmental Microbiology·M C Cirigliano, G M Carman
Dec 1, 1994·The Journal of Allergy and Clinical Immunology·P F Weller, A M Dvorak
Sep 22, 2000·Biochimica Et Biophysica Acta·D ZweytickG Daum
Feb 19, 2002·Bioresource Technology·Seraphim Papanikolaou, George Aggelis
Oct 24, 2002·Archives of Biochemistry and Biophysics·Yi Shan LuoThierry Chardot
Apr 19, 2003·FEMS Yeast Research·Jean-Marc NicaudClaude Gaillardin
May 28, 2003·Applied Microbiology and Biotechnology·P FickersP Thonart

❮ Previous
Next ❯

Citations

Jan 13, 2006·Applied Microbiology and Biotechnology·Andreas ShiningavamweMartie Smit
Jul 20, 2006·Applied Microbiology and Biotechnology·Stylianos FakasGeorge Aggelis
Aug 12, 2009·Applied Microbiology and Biotechnology·Ashok V BankarSmita S Zinjarde
Oct 29, 2010·Applied Microbiology and Biotechnology·Cynthia Romero-GuidoYves Waché
Apr 1, 2011·Applied Microbiology and Biotechnology·Athanasios BeopoulosClaude Gaillardin
Jul 4, 2013·Applied Microbiology and Biotechnology·Bao Ngoc Pham-HoangYves Waché
Oct 19, 2013·Applied Microbiology and Biotechnology·A BeopoulosJean-Marc Nicaud
Sep 6, 2003·Methods : a Companion to Methods in Enzymology·Sabine HovesMartin Fleck
Jun 1, 2007·Molecular Biology of the Cell·René BartzPingsheng Liu
Mar 1, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Philomène KabranCécile Neuvéglise
Oct 28, 2008·Applied and Environmental Microbiology·Athanasios BeopoulosJean-Marc Nicaud
Apr 12, 2011·Eukaryotic Cell·Mykhaylo O DebelyyRalf Erdmann
May 15, 2013·PloS One·Stéphanie MichelyCécile Neuvéglise
Jul 17, 2015·Critical Reviews in Biotechnology·Kyle V ProbstPraveen V Vadlani
Aug 8, 2015·Biotechnology Advances·Hu-Hu LiuHe Huang
Dec 26, 2015·Progress in Lipid Research·Rodrigo Ledesma-Amaro, Jean-Marc Nicaud
Mar 2, 2010·New Biotechnology·Lu-Te ChuangYung-Sheng Huang
Sep 2, 2009·Progress in Lipid Research·Athanasios BeopoulosJean-Marc Nicaud
Sep 1, 2015·Current Opinion in Biotechnology·Quinn Zhu, Ethel N Jackson
Aug 28, 2007·Bioresource Technology·C AngerbauerG M Guebitz
Oct 14, 2014·Metabolic Engineering·Zbigniew LazarJean-Marc Nicaud
Jan 22, 2005·Molecular Microbiology·Marc WältermannAlexander Steinbüchel
Oct 26, 2012·Biotechnology and Bioengineering·Zhenlin HanWei Wen Su
Oct 6, 2012·Yeast·Jean-Marc Nicaud
Dec 18, 2014·MicrobiologyOpen·Rémi DulermoJean-Marc Nicaud
Apr 14, 2016·Biochimica Et Biophysica Acta·Rémi DulermoJean-Marc Nicaud
May 21, 2013·Progress in Lipid Research·Ming-Hua Liang, Jian-Guo Jiang
Apr 23, 2013·Journal of Biotechnology·John BlazeckHal S Alper

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.