Lipid Analysis of Airway Epithelial Cells for Studying Respiratory Diseases

Chromatographia
Nicole ZehethoferPeter König

Abstract

Airway epithelial cells play an important role in the pathogenesis of inflammatory lung diseases such as asthma, cystic fibrosis and COPD. Studies concerning the function of the lipid metabolism of the airway epithelium are so far based only on the detection of lipids by immunohistochemistry but quantitative analyses have not been performed. Although recent advances in mass spectrometry have allowed to identify a variety of lipid classes simultaneously in isolated tissue samples, up until now, these methods were not suitable to analyze lipids in the airway epithelium. To determine all major lipid classes in airway epithelial cells, we used an LC-MS-based approach that can easily be combined with the specific isolation procedure to obtain epithelial cells. We tested the suitability of this method with a mouse model of experimental asthma. In response to allergen challenge, perturbations in the sphingolipids were detected, which led to increased levels of ceramides. We expanded the scope of this approach analysing human bronchus samples without pathological findings of adenocarcinoma patients. For the human lung epithelium an unusual lipid class distribution was found in which ceramide was the predominant sphingolipid. In summary...Continue Reading

References

Nov 10, 2000·Journal of the American Society for Mass Spectrometry·J Zhu, R B Cole
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·K Simons, D Toomre
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Nov 29, 2007·The Journal of Pharmacology and Experimental Therapeutics·Emanuela MasiniDaniela Salvemini
Feb 19, 2008·Journal of Lipid Research·Vitali MatyashDominik Schwudke
Aug 30, 2008·American Journal of Respiratory and Critical Care Medicine·Stefan Uhlig, Erich Gulbins
Dec 4, 2008·Journal of Lipid Research·Robert C MurphyRobert M Barkley
Apr 4, 2009·American Journal of Respiratory and Critical Care Medicine·Thomas RoederMichael Kabesch
Aug 12, 2009·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Akhlaq A Farooqui
Sep 19, 2009·Methods in Molecular Biology·Hiroki NakanishiRyo Taguchi
Oct 10, 2009·Journal of Mass Spectrometry : JMS·Göran HübnerBuko Lindner
Feb 26, 2010·Nature·David K BreslowJonathan S Weissman
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Julio L SampaioAndrej Shevchenko
Mar 16, 2011·Analytical Biochemistry·David G McLarenThomas P Roddy
May 7, 2011·Nature Reviews. Immunology·Sarah Spiegel, Sheldon Milstien
May 26, 2011·Cold Spring Harbor Perspectives in Biology·Dominik SchwudkeAndrej Shevchenko
Jun 17, 2011·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Zhe ZhouMarcus A Mall
Aug 13, 2011·Biochimica Et Biophysica Acta·Valerie B O'Donnell
Sep 9, 2011·Therapeutic Advances in Respiratory Disease·Yang Yang, Stefan Uhlig
Jan 25, 2012·PloS One·Ronny HerzogAndrej Shevchenko
Aug 7, 2012·Molecular Systems Biology·Maria CarvalhoAndrej Shevchenko
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marina MillerDavid H Broide
Sep 29, 2012·Respiration; International Review of Thoracic Diseases·Maria C ScarpaPiero Maestrelli
Apr 10, 2013·Current Opinion in Pharmacology·Malcolm Ronald StarkeyPhilip Michael Hansbro
May 24, 2013·Science Translational Medicine·Tilla S WorgallStefan Worgall
Jul 6, 2013·Cell·Adolfo García-Sastre
Sep 6, 2013·The New England Journal of Medicine·Bruce D Levy
Jan 29, 2014·American Journal of Respiratory Cell and Molecular Biology·Saskia BermbachPeter König
Apr 24, 2014·Expert Opinion on Drug Discovery·Michael FrickerPhilip M Hansbro
Jun 6, 2014·Nature·Michael Maceyka, Sarah Spiegel
Jul 22, 2014·Journal of Cellular Biochemistry·David G ChapmanCharles G Irvin

❮ Previous
Next ❯

Citations

Sep 24, 2015·Journal of Separation Science·Li YangHuwei Liu
Apr 21, 2016·Critical Care Medicine·Sophie NeugebauerMichael Kiehntopf
Feb 16, 2017·American Journal of Respiratory Cell and Molecular Biology·Zhiqiang JiangXiaobo Zhou
Mar 11, 2017·Journal of Lipid Research·Karin A Zemski BerryRobert J Mason
Sep 24, 2016·Respiratory Research·Fatemeh MoheimaniDarryl A Knight
Aug 25, 2020·Lipids in Health and Disease·Lingling XuanLihong Liu
Aug 14, 2020·International Journal of Molecular Sciences·Elena BargagliClaudia Landi
Jan 5, 2021·Nature Biomedical Engineering·Andrew W SimonsonScott H Medina
Oct 11, 2020·EBioMedicine·Clarisse Vandebrouck, Thierry Ferreira
Jun 3, 2021·International Journal of Molecular Sciences·Mayes Alswady-HoffShanbeh Zienolddiny
Jul 3, 2021·Metabolites·Soyoon SimHae-Sim Park
Apr 22, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Saeed NazemidashtarjandiAmir M Farnoud

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
scanning
laser capture microdissection

Software Mentioned

ImageJ
LipidXplorer
GraphPad Prism
Bruker Data Analysis
GraphPad
HyStar

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Related Papers

American Journal of Respiratory and Critical Care Medicine
Malcolm BrodlieChristopher Ward
Handbook of Experimental Pharmacology
Irina Petrache, Daniela Petrusca
© 2022 Meta ULC. All rights reserved