PMID: 9171384Jun 1, 1997Paper

Lipid and fatty acid composition of cytoplasmic membranes from Streptomyces hygroscopicus and its stable protoplast-type L form

Journal of Bacteriology
C HoischenJ Gumpert

Abstract

The cells of an L-form strain of Streptomyces hygroscopicus have been grown for 20 years without a cell wall. Their cytoplasmic membranes have high stability and an unusual structural polymorphism. To clarify the importance of the lipid components for these membrane properties, a comparative analysis has been carried out with purified membranes of L-form cells, of parent vegetative hyphal cells (N-form cells), and of protoplasts derived from the latter. The phospholipid classes and fatty acids were determined by thin-layer chromatography (TLC), two-dimensional TLC, high-performance liquid chromatography, gas chromatography, and mass spectrometry. The qualitative compositions of cardiolipin (CL), lyso-cardiolipin (LCL), phosphatidylethanolamine (PE1 and PE2), lyso-phosphatidylethanolamine (LPE), phosphatidylinositolmannoside (PIM), phosphatidic acid (PA), dilyso-cardiolipin-phosphatidylinositol (DLCL-PI), and the 13 main fatty acids were the same in the three membrane types. However, significant quantitative differences were observed in the L-form membrane. They consist of a three- to fourfold-higher content of total, extractable lipids, 20% more phospholipids, an increased content of CL and PIM, and a reduced amount of the comp...Continue Reading

References

Apr 1, 1978·Chemistry and Physics of Lipids·S G Batrakov, L D Bergelson
Dec 1, 1979·Journal of Bacteriology·M HayamiY Kanemasa
Jan 1, 1982·Zeitschrift für allgemeine Mikrobiologie·J Gumpert
Oct 1, 1980·Archives of Microbiology·H P KrollH H Martin
Dec 1, 1995·Archives of Microbiology·N M Packter, E R Olukoshi
Jan 1, 1996·Journal of Basic Microbiology·J GumpertC Hoischen
Feb 1, 1995·Chemistry & Biology·P A Janmey
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Apr 1, 1965·Journal of Bacteriology·J A NESBITT, W J LENNARZ

❮ Previous
Next ❯

Citations

Mar 14, 2012·Molecular Microbiology·Vinod JyothikumarPaul R Herron
Apr 12, 2001·Journal of Applied Microbiology·C M Innes, E J Allan
Jan 13, 2015·Applied Microbiology and Biotechnology·Lingjun YuGang Liu
Nov 20, 2016·Journal of Industrial Microbiology & Biotechnology·Sean O'RourkeMervyn Bibb
Jan 7, 2016·Frontiers in Microbiology·Mario Sandoval-CalderónChristian Sohlenkamp
Apr 9, 2015·Microbial Biotechnology·Hildgund Schrempf, Philipp Merling
Feb 1, 2015·Applied Microbiology and Biotechnology·Tatjana Ilic-TomicNiko S Radulović
May 15, 2009·The Journal of Biological Chemistry·Mario Sandoval-CalderónChristian Sohlenkamp
Sep 24, 2016·Pharmaceuticals·Nermina Malanovic, Karl Lohner
Mar 4, 2008·Applied and Environmental Microbiology·Ana ArabolazaHugo Gramajo
Feb 2, 2002·Applied and Environmental Microbiology·Christian HoischenBeatrix Fahnert
Oct 16, 2020·Protein Science : a Publication of the Protein Society·Karolina Corin, James U Bowie

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.