Lipid Bilayers Manipulated through Monolayer Technologies for Studies of Channel-Membrane Interplay

Biological & Pharmaceutical Bulletin
Shigetoshi Oiki, Masayuki Iwamoto

Abstract

Fluidity and mosaicity are two critical features of biomembranes, by which membrane proteins function through chemical and physical interactions within a bilayer. To understand this complex and dynamic system, artificial lipid bilayer membranes have served as unprecedented tools for experimental examination, in which some aspects of biomembrane features have been extracted, and to which various methodologies have been applied. Among the lipid bilayers involving liposomes, planar lipid bilayers and nanodiscs, recent developments of lipid bilayer methods and the results of our channel studies are reviewed herein. Principles and techniques of bilayer formation are summarized, which have been extended to the current techniques, where a bilayer is formed from lipid-coated water-in-oil droplets (water-in-oil bilayer). In our newly developed method, termed the contact bubble bilayer (CBB) method, a water bubble is blown from a pipette into a bulk oil phase, and monolayer-lined bubbles are docked to form a bilayer through manipulation by pipette. An asymmetric bilayer can be readily formed, and changes in composition in one leaflet were possible. Taking advantage of the topological configuration of the CBB, such that the membrane's hyd...Continue Reading

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Citations

Dec 5, 2018·Proceedings of the National Academy of Sciences of the United States of America·Masayuki Iwamoto, Shigetoshi Oiki
Mar 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kenichiro MitaShigetoshi Oiki

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