PMID: 4366759Jun 1, 1974

Lipid binding to the amphipathic membrane protein cytochrome b5

Proceedings of the National Academy of Sciences of the United States of America
P J DehlingerO H Griffith


The lipid binding properties of the membrane protein cytochrome b(5) (detergent-extracted from calf liver microsomal preparations) were characterized by studying the interaction of spin-labeled lipids (5-, 12-, and 16-doxylstearic acid and 5- and 16-doxylphosphatidyl-choline, where doxyl refers to the nitroxide moiety) with cytochrome b(5), using electron spin resonance spectroscopy. The intact cytochrome b(5) molecule immobilizes all of the lipid spin labels, while the segment of cytochrome b(5) released by trypsin does not affect lipid mobility. The immobilization of lipid spin labels on the hydrophobic surface of intact cytochrome b(5) is not appreciably altered by associating the protein with liposomes. Differences in polarity of the lipid binding sites between cytochrome b(5) and phospholipid vesicles were also observed. The lipid binding sites on cytochrome b(5) are hydrophobic by conventional criteria, but are more polar than the interior of fluid phospholipid bilayers.


Jul 14, 1971·Journal of Molecular Biology·P JostO H Griffith
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·P JostG Vanderkooi
Jan 1, 1973·Journal of Supramolecular Structure·P JostO H Griffith
Dec 31, 1973·Annals of the New York Academy of Sciences·O H GriffithG Vanderkooi
Oct 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Dallner, L Ernster
May 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·L Spatz, P Strittmatter
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·A TsugitaM A Rashid
Jan 27, 1971·Journal of the American Chemical Society·W L Hubbell, H M McConnell


Jan 1, 1976·Journal of Supramolecular Structure·W H Huestis
Jan 1, 1979·Journal of Supramolecular Structure·A Y RomansC M Grisham
Feb 6, 1978·The Journal of Membrane Biology·J M Boggs, M A Moscarello
Jun 14, 1990·Biochimica Et Biophysica Acta·P BanerjeeG Dawson
Apr 28, 1995·Biochimica Et Biophysica Acta·A Leikin, M Shinitzky
Dec 29, 1975·Biochimica Et Biophysica Acta·J W Depierre, G Dallner
Aug 15, 1975·Biochimica Et Biophysica Acta·J M Anderson
Sep 18, 2001·European Journal of Biochemistry·P Hlavica, D F Lewis
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C W Grant, H M McConnell
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E OldfieldD M Rice
Apr 10, 1978·Biochimica Et Biophysica Acta·J M Boggs, M A Moscarello
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Oct 26, 1976·Biochimica Et Biophysica Acta·C Tanford, J A Reynolds
Jan 1, 1979·CRC Critical Reviews in Biochemistry·E J PatzerE J Dubovi
Dec 27, 1996·Annals of the New York Academy of Sciences·W W Just, P Diestelkötter

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