PMID: 39617Aug 30, 1979

Lipid composition of aminopterin-resistant and sensitive strains of Streptococcus pneumoniae. Effect of aminopterin inhibition

Biochimica Et Biophysica Acta
M C TrombeG Laneelle

Abstract

The polar lipids of Streptococcus pneumoniae wild type and aminopterin-resistant strains were analysed. The membrane contained only two acid phospholipids, phosphatidylglycerol and cardiolipin, and a large amount of two glycolipids, glucosyldiglyceride and galactosylglucosyldiglyceride. The unsaturated acyl chains ranged from 58 to 87% of total fatty acids, depending on the strain and on growth conditions. No relation could be established between aminopterin resistance and polar lipid or fatty acid compositions. However, in the presence of bacteriostatic concentrations of aminopterin, the wild type and the resistant mutant did not have the same behavior. The resistant strain maintained its fatty acid composition and a normal [32P]phosphate distribution among phospholipids while the wild type shifted to a higher content in unsaturated fatty acids and to a high relative cardiolipin labelling. Such a differencein [32P] distribution was not observed when bacteriostatic concentrations of chloramphenicol were used, or when growth was stopped after amino acid deprivation induced by high concentrations of isoleucine. The biochemical basis of the aminopterin resistant character of the amiA mutants are not yet well understood but the pre...Continue Reading

References

Dec 5, 2006·Journal of Bacteriology·Jason W RoschMichael G Caparon
Aug 30, 2014·Biochimica Et Biophysica Acta·Ségolène LaageAnn E McDermott
Apr 12, 2015·FEMS Microbiology Reviews·Christian Sohlenkamp, Otto Geiger
Jun 30, 2007·Biochimica Et Biophysica Acta·Raquel F EpandRichard M Epand
Mar 31, 2016·Applied Microbiology and Biotechnology·Ti-Yu Lin, Douglas B Weibel
Sep 23, 2016·Pharmaceuticals·Nermina Malanovic, Karl Lohner
Sep 19, 2002·The Journal of Biological Chemistry·Hedia MarrakchiCharles O Rock
Apr 3, 2019·Frontiers in Microbiology·Philippe CalvezAndré Zapun
Sep 11, 2019·Journal of Bacteriology·Luke R JoyceKelli L Palmer
Feb 21, 2018·The Journal of Biological Chemistry·Yu ChenTom A Rapoport

Citations

Jan 1, 1975·Annual Review of Biochemistry·D F Silbert
Mar 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·C B Hirschberg, E P Kennedy
Mar 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·L S Milner, H R Kaback

Related Concepts

Antibiotic Resistance, Microbial
Phosphatidyl glycerol
Phosphatidylglycerols
Glycolipids
Phosphatidylglycerol Measurement
Phosphate Measurement
Saturated Fat
Cardiolipins
Isoleucine Measurement
Metabolic Inhibition

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