Lipid composition of isolated epiphyseal cartilage cells, membranes and matrix vesicles.

Biochimica Et Biophysica Acta
Roy E Wuthier

Abstract

1. Intact cells, cell fragments (membranes) and matrix vesicles were isolated from the proliferating and calcifying layers of epiphyseal cartilage by sequential hyaluronidase and collagenase digestion and differential centrifugation. Lipids were extracted and analyzed for various lipid classes and their fatty acid composition by column, thin-layer, paper and gas-liquid chromatography. 2. On a protein basis the isolated matrix vesicles had more total lipid than either the membrane or cell fractions, the vesicles and membranes being richer in non-polar lipids and containing smaller quantities of phospholipids than whole cells. Expressed as a percentage of the total lipid, the cells were richer in triacylglycerols and lower in free fatty acids than in the membrane or vesicle fractions. The proportion of free cholesterol and the cholesterol/phospholipid ratio were nearly twice as high in the matrix vesicles as in the other tissue fractions. Choline and ethanolamine phosphoglycerides progressively declined in the membrane and matrix vesicle fractions, whereas serine phosphoglycerides and sphinogomyelin increased. Non-phosphorus-containing polar lipids were present in all fractions, the vesicles being richer in polyhexosyl ceramides...Continue Reading

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Citations

Jul 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B R GengeR E Wuthier
Nov 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R J LeachB D Boyan
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May 13, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J LiberaA Herrmann
Jul 2, 2014·Journal of Materials Science. Materials in Medicine·Bérengère J C LuthringerRegine Willumeit
Dec 9, 2010·Seminars in Immunopathology·Ellis E Golub
Sep 30, 2009·Biochimica Et Biophysica Acta·Ellis E Golub
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Dec 21, 2007·Proteomics·Marcin BalcerzakSlawomir Pikula
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May 1, 1992·Bone and Mineral·E D Eanes

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