Lipid deprivation increases surfactant phosphatidylcholine synthesis via a sterol-sensitive regulatory element within the CTP:phosphocholine cytidylyltransferase promoter

The Biochemical Journal
Rama K MallampalliChristie P Thomas

Abstract

Lipid-deprived mice increase alveolar surfactant disaturated phosphatidylcholine (DSPtdCho) synthesis compared with mice fed a standard diet by increasing expression of CTP:phosphocholine cytidylyltransferase (CCT), the rate-limiting enzyme for DSPtdCho synthesis. We previously observed that lipid deprivation increases mRNA synthesis for CCT [Ryan, McCoy, Mathur, Field and Mallampalli (2000) J. Lipid Res. 41, 1268-1277]. To evaluate regulatory mechanisms for this gene, we cloned the proximal approximately 1900 bp of the 5' flanking sequence of the murine CCT gene, coupled this to a luciferase reporter, and examined transcriptional regulation in a murine alveolar epithelial type II cell line (MLE-12). The core promoter was localized to a region between -169 and +71 bp, which exhibited strong basal activity comparable with the simian virus 40 promoter. The full-length construct, from -1867 to +71, was induced 2-3-fold when cells were cultured in lipoprotein-deficient serum (LPDS), similar to the level of induction of the endogenous CCT gene. By deletional analysis the sterol regulatory element (SRE) was localized within a 240 bp region. LPDS activation of the CCT promoter was abolished by mutation of this SRE, and gel mobility-sh...Continue Reading

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Citations

Jul 5, 2003·Biochimica Et Biophysica Acta·Mohammad KarimSuzanne Jackowski
Jun 28, 2003·American Journal of Respiratory Cell and Molecular Biology·Jiming ZhouRama K Mallampalli
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jean E Vance, Dennis E Vance
Nov 13, 2004·The Journal of Biological Chemistry·Suzanne Jackowski, Paolo Fagone
Feb 26, 2008·Progress in Lipid Research·Hiroyuki SugimotoDennis E Vance
Oct 3, 2012·Biochimica Et Biophysica Acta·Marianna Agassandian, Rama K Mallampalli

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