Lipid diffusion from single molecules of a labeled protein undergoing dynamic association with giant unilamellar vesicles and supported bilayers

Langmuir : the ACS Journal of Surfaces and Colloids
Alexey SharonovRobin M Hochstrasser

Abstract

It is demonstrated that single-molecule tracking of a fluorescently labeled protein undergoing transient binding to model membranes presents a useful method of obtaining fluid properties. The labeled ACBP protein was tracked during its binding to free-standing giant unilamellar vesicles (GUVs) and supported bilayers prepared from the GUVs in the same environment. The analysis of images that are blurred as a result of fast probe diffusion was discussed. An examination of the lateral diffusion trajectories revealed a homogeneous diffusion on the top segments of the GUVs with D = 6.9 +/- 0.3 microm(2)/s. The supported bilayer experiments revealed two diffusion processes, one with Df = 3.1 +/- 0.4 microm(2)/s and the other with Ds = 0.078 +/- 0.001 microm(2)/s. The 2-fold difference in the lipid bilayer mobility for the free-standing and fast components in the supported bilayers is attributed to the known effect of frictional coupling with the solid support. The slow mobile fraction in the bilayer is suggested to be associated with the migration of pore-like structures, originating from the interaction of the membrane with the glass support.

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Citations

Apr 14, 2010·International Journal of Molecular Sciences·Radek Machán, Martin Hof
Jun 5, 2014·ACS Applied Materials & Interfaces·Paul KühlerTheobald Lohmüller
Aug 3, 2011·The Journal of Physical Chemistry. B·Amit Kumar MandalKankan Bhattacharyya
Apr 5, 2012·The Journal of Physical Chemistry. B·Sharon RozovskyJay T Groves
Jul 28, 2009·Current Opinion in Chemical Biology·Anne Gershenson
Oct 15, 2013·Environmental Microbiology·Ariadna MartosPetra Schwille
Mar 2, 2010·Biochimica Et Biophysica Acta·Radek Machán, Martin Hof
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Oct 3, 2009·Biochimica Et Biophysica Acta·Marie-Cécile GiocondiChristian Le Grimellec
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Sep 1, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·N P W PieczonkaR F Aroca

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