Lipid Exchange Factors at Membrane Contact Sites in African Swine Fever Virus Infection

Viruses
Inmaculada GalindoC Alonso

Abstract

African swine fever (ASF) is a hemorrhagic fever of wild and domestic pigs with a high rate of mortality. Originally endemic in Africa, this disease is currently disseminating in Europe and China, causing a large socioeconomic impact. ASF is caused by a DNA virus, African swine fever virus (ASFV). There is no vaccine available against ASFV, limiting the options for disease control. ASFV reorganizes intracellular membranes to generate viral factories (VFs) in order to amplify its genome. However, little is known about the process involved in the formation of these viral replication organelles. Membrane contact sites (MCSs) allow nonvesicular lipids and ion exchange between organelles. Lipid exchange to form VFs apparently requires a number of proteins at MCSs, such as the oxysterol-binding protein (OSBP), the acyl-coenzyme A binding domain containing 3 (ACBD3) and the phosphatidylinositol-phosphate-4-kinase III beta (PI4Kβ). Itraconazole (ITZ) is an antifungal agent that targets sterol-transport molecules such as OSBP and OSBP-related protein 4 (ORP4). 25-Hydroxycholesterol (25-HC) inhibits lipid transport by high affinity binding OSBP. In this work, we analyzed the antiviral function of ITZ and 25-HC against ASFV in Vero cell c...Continue Reading

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Citations

Sep 20, 2019·Viruses·Axel KargerMaria Montoya
Apr 22, 2021·Research in Veterinary Science·Fengxue WangYongjun Wen
Jul 13, 2021·Frontiers in Microbiology·Kiran AvulaGulam H Syed

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Methods Mentioned

BETA
FACS
Assay
PCR
fluorescence microscopy
flow cytometry

Software Mentioned

Image lab
Leica Application Suite
ImageJ
GraphPad Prism

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