Lipid-free antigen B subunits from echinococcus granulosus: oligomerization, ligand binding, and membrane interaction properties

PLoS Neglected Tropical Diseases
Valeria Silva-ÁlvarezBetina Corsico

Abstract

The hydatid disease parasite Echinococcus granulosus has a restricted lipid metabolism, and needs to harvest essential lipids from the host. Antigen B (EgAgB), an abundant lipoprotein of the larval stage (hydatid cyst), is thought to be important in lipid storage and transport. It contains a wide variety of lipid classes, from highly hydrophobic compounds to phospholipids. Its protein component belongs to the cestode-specific Hydrophobic Ligand Binding Protein family, which includes five 8-kDa isoforms encoded by a multigene family (EgAgB1-EgAgB5). How lipid and protein components are assembled into EgAgB particles remains unknown. EgAgB apolipoproteins self-associate into large oligomers, but the functional contribution of lipids to oligomerization is uncertain. Furthermore, binding of fatty acids to some EgAgB subunits has been reported, but their ability to bind other lipids and transfer them to acceptor membranes has not been studied. Lipid-free EgAgB subunits obtained by reverse-phase HPLC were used to analyse their oligomerization, ligand binding and membrane interaction properties. Size exclusion chromatography and cross-linking experiments showed that EgAgB8/2 and EgAgB8/3 can self-associate, suggesting that lipids are ...Continue Reading

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Citations

Feb 6, 2016·Parasites & Vectors·Valeria Silva-ÁlvarezAna María Ferreira
Oct 2, 2015·Parasite Immunology·A DíazA M Ferreira
Nov 18, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Daniela PagnozziSergio Uzzau
Jul 22, 2018·Parasite Immunology·Valeria Silva-ÁlvarezAna M Ferreira
May 5, 2018·PLoS Neglected Tropical Diseases·Edileuza Danieli da SilvaArnaldo Zaha

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Datasets Mentioned

BETA
AAQ74958
BAE94835

Methods Mentioned

BETA
size exclusion chromatography
electrophoresis
circular dichroism
Fluorescence
FRET

Software Mentioned

SigmaPlot
Solver Add - In
Biology Workbench
DrawTree
ClustalW
GraphPad Prism
Excel

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