Oct 27, 1999

Lipid-induced changes in vascular smooth muscle cell membrane fluidity are associated with DNA synthesis

Cell Proliferation
A SachinidisH Vetter

Abstract

In the present study, we examined whether changes in the membrane fluidity of vascular smooth muscle cells (VSMCs) alter their DNA synthesis. For this purpose, the membrane fluidity of the cells was modulated after treatment of VSMCs with 1,2-dioleoyl phosphatidylcholine (PC). Treatment of VSMCs with 1,2-dioleoyl PC-rich medium containing 10% heat-inactivated human serum and 3 mg/ ml 1,2-dioleoyl PC for 24 h resulted in an increase in VSMC membrane fluidity at all temperatures from 15 degrees to 40 degrees C as well as a 51% inhibition of DNA synthesis, compared with untreated cells. Remarkably, enrichment of VSMCs with 1,2-dioleoyl PC/cholesterol-rich medium containing 10% human serum, 3 mg/ml 1,2-dioleoyl PC and 2 mg/ml cholesterol restored both membrane fluidity and DNA synthesis to the levels of untreated cells. The present findings show an inverse association between increased membrane fluidity and cellular DNA synthesis.

  • References7
  • Citations11

Citations

Mentioned in this Paper

Muscle, Smooth, Vascular
Thermodynamics
Epicholesterol
1,2-oleoylphosphatidylcholine
1,2-dioleoyl-sn-glycerol-3-ethylphosphocholine
Rats, Inbred WKY
Phosphatidylcholines
Metabolic Inhibition
DNA Biosynthetic Process
DNA Replication

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