Lipid mediator Leukotriene D4 -induces airway epithelial cells proliferation through EGFR/ERK1/2 pathway

Prostaglandins & Other Lipid Mediators
Neeraj Dholia, Umesh C S Yadav

Abstract

Cysteinyl leukotrienes (CysLTs), the potent lipid inflammatory mediators, are elevated in many pathological conditions and implicated in various inflammatory diseases including asthma, however their role in airway epithelial cells modulation is not clearly understood. We have investigated the effects of a CysLT, Leukotriene D4 (LTD4) on human airway epithelial cells, and assessed its role and mode of action in these cells. Human small airway epithelial cells (SAECs) and A549 cells were incubated with different concentrations of LTD4 for different time intervals. Subsequently trypan blue dye exclusion assay, MTT assay, Western blotting, RT-PCR and immunofluorescence experiments were performed to examine the effects of LTD4 on proliferation and related molecular changes in the airway epithelial cells. The treatment of human airway epithelial cells with LTD4 resulted in a significant increase in cell proliferation and modulation in the expression of receptors, CysLT1R and CysLT2R in SAECs as well as A549 cells. In both types of cells, LTD4 increased the expression levels of PCNA and c-myc, and trans-activated EGF receptor and increased the activation of ERK1/2. When treated along with epidermal growth factor (EGF), LTD4 showed a m...Continue Reading

Citations

May 13, 2020·PPAR Research·Oxana Yu KytikovaTatyana A Gvozdenko
Jun 21, 2019·Medicina·Oxana KytikovaTatyana Gvozdenko
Jul 25, 2019·International Journal of Molecular Sciences·Airi Jo-WatanabeTakehiko Yokomizo
Nov 3, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Neeraj DholiaUmesh C S Yadav
Jan 30, 2021·Obesity Research & Clinical Practice·Jiten R Sharma, Umesh C S Yadav

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