Lipid microdomain formation: characterization by infrared spectroscopy and ultrasonic velocimetry

Biophysical Journal
Zachary D Schultz, Ira W Levin

Abstract

We demonstrate the use of vibrational infrared spectroscopy applied to characterize lipid microdomain sizes derived from a model raft-like system consisting of nonhydroxy galactocerebroside, cholesterol, and dipalmitoylphosphatidylcholine components. The resulting spectroscopic correlation field components of the lipid acyl chain CH(2) methylene deformation modes, observed when lipid multilamellar assemblies are rapidly frozen from the liquid crystalline state to the gel phase, indicate the existence of lipid microdomains on a scale of several nanometers. The addition of cholesterol disrupts the glycosphingolipid selectively but perturbs the di-saturated chain phospholipid matrix. Complementary acoustic velocimetry measurements indicate that the microdomain formation decreases the total volume adiabatic compressibilities of the multilamellar vesicle assemblies. The addition of cholesterol, however, disrupts the galactocerebroside domains, resulting in a slight increase in the lipid assemblies' total adiabatic compressibility. The combination of these two physical approaches offers new insight into microdomain formation and their properties in model bilayer systems.

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Citations

Jul 17, 2009·The Journal of Physical Chemistry. B·Zachary D SchultzIra W Levin
Apr 5, 2011·Annual Review of Analytical Chemistry·Zachary D Schultz, Ira W Levin
Mar 16, 2016·International Journal of Pharmaceutics·Giulia BonacucinaGiovanni F Palmieri
Mar 24, 2016·Biochimica Et Biophysica Acta·Sanja Pöyry, Ilpo Vattulainen
Dec 17, 2008·Biochimica Et Biophysica Acta·Bodil Westerlund, J Peter Slotte
Oct 27, 2012·Biochimica Et Biophysica Acta·Ruthven N A H Lewis, Ronald N McElhaney
Aug 6, 2013·Biochimica Et Biophysica Acta·Hui-Hsu Gavin TsaiJian-Bin Lee
Dec 1, 2009·Biochimica Et Biophysica Acta·Marjorie L Longo, Craig D Blanchette
Aug 6, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Jay P Kitt, Joel M Harris
Oct 18, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Tarek Samir AwadDavid Julian McClements
Mar 28, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·David A BryceJoel M Harris
Oct 4, 2011·The Journal of Physical Chemistry. B·Carme Suárez-GermàOscar Domènech

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