Lipid nanoparticle technologies for the study of G protein-coupled receptors in lipid environments

Biophysics Reviews
Steven Lavington, Anthony Watts

Abstract

G protein-coupled receptors (GPCRs) are a large family of integral membrane proteins which conduct a wide range of biological roles and represent significant drug targets. Most biophysical and structural studies of GPCRs have been conducted on detergent-solubilised receptors, and it is clear that detergents can have detrimental effects on GPCR function. Simultaneously, there is increasing appreciation of roles for specific lipids in modulation of GPCR function. Lipid nanoparticles such as nanodiscs and styrene maleic acid lipid particles (SMALPs) offer opportunities to study integral membrane proteins in lipid environments, in a form that is soluble and amenable to structural and biophysical experiments. Here, we review the application of lipid nanoparticle technologies to the study of GPCRs, assessing the relative merits and limitations of each system. We highlight how these technologies can provide superior platforms to detergents for structural and biophysical studies of GPCRs and inform on roles for protein-lipid interactions in GPCR function.

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Citations

Dec 9, 2020·Biophysics Reviews·Damien Hall
May 13, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Victoria Ariel BjørnestadReidar Lund
Jun 3, 2021·Biomolecules·Matthias Elgeti, Wayne L Hubbell

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Methods Mentioned

BETA
nuclear
electron paramagnetic resonance
microscale
NMR
nucleotide exchange
circular dichroism
SMA
size exclusion chromatography
PMA
fluorescence correlation spectroscopy

Software Mentioned

DEER

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