PMID: 2872199May 1, 1986

Lipid peroxidation and activities of tyrosine aminotransferase and glutamine synthetase in hepatoma and glioma cells grown in bovine colostrum-supplemented medium

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
L OdlandErik Walum

Abstract

The growth stimulating properties of bovine serum and colostrum were compared in rat hepatoma (HTC) and glioma (C6) cell cultures. A colostrum concentration of 2% was optimal for HTC cells, which then reached a terminal density 40% of that in serum-supplemented medium. The corresponding figures for C6 cells were 10 and 81%, respectively. After 4 d in culture, levels of lipid hydroperoxides were measured and compared. Highest levels of lipid hydroperoxides were found in HTC and C6 cells grown in unsupplemented medium. HTC and C6 cells grown in serum supplemented medium contained levels of 52 and 64%, respectively, of that in unsupplemented medium. The corresponding levels for cells grown in presence of colostrum were 40% for HTC and 44% for C6 cells. To obtain information on any functional alterations in the cells due to the presence of colostrum the induction of tyrosine aminotransferase (EC 2.6.1.5) and glutamine synthetase (EC 6.3.1.2) by dexamethasone was studied. Although colostrum seemed to increase the basal activities of the enzymes, no significant effects on the degree of induction could be detected.

References

Sep 1, 1979·Molecular and Cellular Endocrinology·E B ThompsonG L Hager
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Klagsbrun
Jan 1, 1979·Methods in Enzymology·E B Thompson
Aug 1, 1979·Archives of Biochemistry and Biophysics·A S Giasuddin, A T Diplock
Jan 1, 1979·Journal of Supramolecular Structure·M Klagsbrun, J Neumann
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·E B ThompsonJ F Curran
Jun 14, 1972·Journal of Molecular Biology·G G RousseauG M Tomkins
Jul 26, 1968·Science·P BendaW Sweet
Jan 1, 1971·Enzyme·J Thorndike, L Reif-Lehrer
Oct 1, 1980·Experimental Cell Research·J E Bottenstein, G H Sato

Citations

Feb 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G K InnesK E Hobbs
Jun 10, 2011·Biochemical and Biophysical Research Communications·Atul Kumar Pandey, Santosh Kumar Kar
Nov 12, 2009·Sports Medicine·Cecilia M ShingLesley M Stevenson
Jul 27, 2007·Alternatives to Laboratory Animals : ATLA·Lars Adamson, Erik Walum

Related Concepts

Metazoa
Blood
Bos indicus
Cell Density
Cell Division Phases
Colostrum
Oradexon
Enzyme Induction
Mixed Gliomas
Glutamate-Ammonia Ligase

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