Lipid peroxidation and apoptotic response in rat brain areas induced by long-term administration of nandrolone: the mutual crosstalk between ROS and NF-kB

Journal of Cellular and Molecular Medicine
Emanuela TurillazziV Fineschi

Abstract

The aim of this study was to evaluate the played by oxidative stress in the apoptotic response in different brain areas of rats chronically treated with supra-physiological doses of nandrolone decanoate (ND). Immunohistochemical study and Western blot analysis were performed to evaluate cells' apoptosis and to measure the effects of expression of specific mediators, such as NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells), Bcl-2 (B-cell lymphoma 2), SMAC/DIABLO (second mitochondria-derived activator of caspases/direct IAP-binding protein with low PI) and VMAT2 (vesicular monoamine transporter 2) on apoptosis. The results of the present study indicate that a long-term administration of ND promotes oxidative injury in rat brain specific areas. A link between oxidative stress and NF-κB signalling pathways is supported by our results. In addition to high levels of oxidative stress, we consistently observed a strong immunopositivity to NF-κB. It has been argued that one of the pathways leading to the activation of NF-κB could be under reactive oxygen species (ROS)-mediated control. In fact, growing evidence suggests that although in limited doses, endogenous ROS may play an activating role in NF-κB signalling, ...Continue Reading

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Citations

Aug 17, 2016·International Journal of Molecular Sciences·Silvia GrottelliIlaria Bellezza
Mar 1, 2017·Journal of Cellular and Molecular Medicine·Rosario BaroneCristoforo Pomara
Jan 26, 2018·Current Opinion in Endocrinology, Diabetes, and Obesity·Gen KanayamaHarrison G Pope
Sep 29, 2020·Journal of Translational Internal Medicine·Pengbo WangYingxian Sun
Nov 4, 2017·Oxidative Medicine and Cellular Longevity·Alexandra C Sevastre-BerghianSimona V Clichici
May 3, 2019·Oxidative Medicine and Cellular Longevity·Tingli SunPeilong Ma
May 20, 2020·International Reviews of Immunology·Zhou YangBo Yu
Jan 10, 2018·Frontiers in Molecular Neuroscience·Rodrigo E González-ReyesLaura Mora-Muñoz
Jan 23, 2021·Healthcare·Giuseppe Davide AlbanoAngelo Montana
Nov 15, 2020·Medicina·Federico Giuseppe PatanèAngelo Montana
Feb 8, 2020·Current Neuropharmacology·Shareen Singh, Thakur Gurjeet Singh
Jul 5, 2021·Environmental Science and Pollution Research International·Mohamed SeifZaizhao Wang
Aug 28, 2021·Brain Sciences·Maria Ester La TorreChiara Porro

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Methods Mentioned

BETA
MDA
ubiquitination
nuclear translocation

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