Lipid peroxidation, arachidonic acid and products of the lipoxygenase pathway in ischaemic preconditioning of rat heart

Cardiovascular Research
J StarkopfK Ytrehus

Abstract

Preconditioning with brief intermittent periods of ischaemia is known to provide protection against ischaemic injury. It has been suggested that myocardial ischaemia also activates phospholipase A2, which releases arachidonic acid from phospholipids. In the present study the possible role of phospholipid peroxidation, arachidonic acid and products of the lipoxygenase pathway in cellular mechanisms of ischaemic preconditioning was examined. Isolated, buffer-perfused rat hearts were freeze-clamped at the end of preconditioning (a cycle of 5 min global ischaemia +5 min reperfusion) and at the end of 30 min global ischaemia and analysed for non-esterified fatty acids and fatty acids in the 2-position of phospholipid. In a separate set of experiments, hearts pretreated with a lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA), were subjected to 30 min regional ischaemia and 120 min reperfusion. Infarct size was determined by tetrazolium staining and the ischaemic risk zone with fluorescent particles. Myocardial levels of arachidonic as well as of linoleic and docosahexaenoic acid were significantly elevated by preconditioning. Also, the level of peroxidized polyunsaturated fatty acids (measured as hydroxy conjugated dienes) in...Continue Reading

Citations

Sep 6, 2011·Basic Research in Cardiology·Yumei YeYochai Birnbaum
Jun 13, 2002·Clinical and Experimental Pharmacology & Physiology·Ho Jan HanSung Keel Kang
Apr 28, 2005·Clinical and Experimental Pharmacology & Physiology·Claire ArnaudChristophe Ribuot
Jun 27, 2006·The Journal of Thoracic and Cardiovascular Surgery·J McGuinnessJ M Redmond
Mar 1, 2012·The Biochemical Journal·Ashlee HigdonVictor M Darley-Usmar
Aug 18, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·K Ytrehus
Nov 3, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ru ZhouPing Ma
Nov 28, 2017·Cellular and Molecular Neurobiology·Eric J KnottNicolas G Bazan
Apr 11, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Yumei YeYochai Birnbaum
May 2, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alison SextonAmrita Ahluwalia
Oct 22, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Yumei YeYochai Birnbaum
Apr 12, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S A GabelE Murphy
Jun 25, 2002·Annals of the New York Academy of Sciences·Olaf DransfeldJürgen Eckel
May 2, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Yasuo M TsutsumiDavid M Roth
Mar 25, 2004·Journal of Cell Science·Xupei Huang, Jeffery W Walker
Nov 18, 2000·Biochimica Et Biophysica Acta·S Nigam, T Schewe

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