Lipid peroxidation associated cardiolipin loss and membrane depolarization in rat brain mitochondria

Neurochemistry International
Tanusree SenSasanka Chakrabarti

Abstract

Oxidative stress induced by Fe2+ (50 microM) and ascorbate (2 mM) in isolated rat brain mitochondria incubated in vitro leads to an enhanced lipid peroxidation, cardiolipin loss and an increased formation of protein carbonyls. These changes are associated with a loss of mitochondrial membrane potential (depolarization) and an impaired activity of electron transport chain (ETC) as measured by MTT reduction assay. Butylated hydroxytoluene (0.2 mM), an inhibitor of lipid peroxidation, can prevent significantly the loss of cardiolipin, the increased protein carbonyl formation and the decrease in mitochondrial membrane potential induced by Fe2+ and ascorbate, implying that the changes are secondary to membrane lipid peroxidation. However, iron-ascorbate induced impairment of mitochondrial ETC activity is apparently independent of lipid peroxidation process. The structural and functional derangement of mitochondria induced by oxidative stress as reported here may have implications in neuronal damage associated with brain aging and neurodegenerative disorders.

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Jan 1, 1991·Acta Neurologica Scandinavica. Supplementum·P Jenner
Oct 1, 1990·Photochemistry and Photobiology·C GiuliviA Boveris
Aug 15, 1984·The Biochemical Journal·T F Slater
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M K ShigenagaB N Ames
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·G CohenN Kesler
Mar 31, 1999·Journal of Neurochemistry·M J PickloT J Montine
Oct 26, 1999·Biochemical and Biophysical Research Communications·Y ShidojiK Yagi
May 9, 2001·Biochimica Et Biophysica Acta·M J Picklo, T J Montine
Apr 12, 2003·Progress in Lipid Research·Koji Uchida
Jun 20, 2003·Experimental Gerontology·Hemontika ChakrabortySasanka Chakrabarti
Dec 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Giuseppe PetrosilloGiuseppe Paradies
Apr 24, 2004·Annals of the New York Academy of Sciences·Kazuhiro HondaMark A Smith
Jan 27, 2005·Neurochemistry International·Jian Luo, Riyi Shi

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Citations

Sep 7, 2007·Molecular and Cellular Biochemistry·Martha E Vázquez-MemijeMohammed El-Hafidi
Nov 11, 2009·Biological Trace Element Research·Lin WangZongping Liu
Dec 19, 2012·Antioxidants & Redox Signaling·Tiago R FigueiraAnibal E Vercesi
Apr 17, 2009·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Subhasis DasSomenath Roy
Feb 14, 2007·Journal of Nanobiotechnology·Angela O ChoiDusica Maysinger
Jan 22, 2014·Chemistry and Physics of Lipids·Vaishnavi Raja, Miriam L Greenberg
May 6, 2011·The Journal of Nutritional Biochemistry·Christopher MasterjohnRichard S Bruno
Jun 6, 2009·Ecotoxicology and Environmental Safety·Mariana AstizCarlos Alberto Marra
Sep 10, 2015·Journal of Bioenergetics and Biomembranes·Analía CzerniczyniecS Lores-Arnaiz
Jun 15, 2010·Environmental and Molecular Mutagenesis·James R Roede, Dean P Jones
Oct 10, 2015·Environmental Toxicology·Somaira KhanSheikh Raisuddin
Aug 17, 2006·Neurochemistry International·Panchanan MaitiG Ilavazhagan
Apr 12, 2016·Molecular Nutrition & Food Research·Ryan M BradleyRobin E Duncan
Mar 8, 2008·Free Radical Research·João P SilvaOlga P Coutinho
Aug 11, 2006·American Journal of Physiology. Cell Physiology·Adam J Chicco, Genevieve C Sparagna
Dec 12, 2012·Journal of Applied Physiology·Olga OstojicDavid A Hood
Jan 1, 2017·Cellular and Molecular Neurobiology·Caitlin B Pointer, Andis Klegeris
Jan 15, 2013·Human & Experimental Toxicology·Lin WangZhenyong Wang
Mar 1, 2021·Trends in Endocrinology and Metabolism : TEM·Micol FalabellaRobert D S Pitceathly
Dec 7, 2007·Chemical Research in Toxicology·Lawrence M SayreMark A Smith
Dec 5, 2016·Pesticide Biochemistry and Physiology·Leila HocineMichel Narce

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