PMID: 3125142Feb 1, 1988

Lipid peroxidation in hepatocyte cell cultures: modulation by free radical scavengers and iron

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
G K InnesK E Hobbs

Abstract

Rat hepatocytes were isolated and then maintained in serum-free cell culture medium for 24 h. The amount of malondialdehyde (MDA) accumulated in the medium was assayed and used as a measure of lipid peroxidation. The activity of lactate dehydrogenase (LDH) and urea were measured in the medium and used as indicators of hepatocellular viability and function. The effects of iron; desferrioxamine mesylate (Desferal), an iron chelator; and mannitol, a hydroxyl free radical scavenger were investigated. The addition of iron, Fe2 resulted in a three-fold increase in the levels of MDA. Desferal inhibited the production of MDA and blocked the effect of Fe2+. Neither iron nor Desferal had any effect on LDH or urea levels. Mannitol had no effect on MDA or urea production, but caused a 4 to 8-fold increase in the LDH levels in the medium. The results show that iron is involved in the mechanism of lipid peroxidation in hepatocyte cultures but suggest that as a pathologic event lipid peroxidation is not expressed in terms of viability during the first 24 h of hepatocyte culture.

References

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Citations

Aug 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·F J Darfler
Oct 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·B J Fuller, K Cheetham
Oct 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H BabichA Stern
Jan 30, 2002·Toxicology Letters·Lawrence M Milchak, J Douglas Bricker
May 3, 2001·Journal of Photochemistry and Photobiology. B, Biology·A B PetersenH C Wulf
Mar 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·T P LinS Nandi
Jun 1, 1996·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·A Markant, J Pallauf
Oct 9, 2002·Medicine and Science in Sports and Exercise·Jon LennGeza Bruckner

Related Concepts

Metazoa
Cell Survival
Desferal
Iron
Lactate Dehydrogenase
Lipid Peroxides
Liver
Sodium Malondialdehyde
Osmitrol
Carmol

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