Lipid peroxidation in low density lipoproteins from human plasma and egg yolk promotes accumulation of 1-acyl analogues of platelet-activating factor-like lipids

Lipids
A TokumuraK Fukuzawa

Abstract

Oxidative modification of low density lipoprotein (LDL) is known to be a key event for induction of atherosclerosis. However, there has been little progress in structural elucidation of oxidized lipids, especially oxidatively fragmented phospholipids retaining a glycerol backbone. In this study, we found that LDL derived from egg yolk has no platelet-activating factor (PAF) acetylhydrolase activity, and that prolonged incubation of egg yolk LDL with Cu2+ resulted in the formation of various PAF-like lipids: 1-acyl type phosphatidylcholines with an sn-2-short-chain dicarboxylate or monocarboxylate group. Only a very small amount of the PAF-like lipid having an sn-2-short-chain monocarboxylate group was detected by gas chromatography-mass spectrometry in Cu(2+)-oxidized LDL from human plasma with high PAF-acetylhydrolase activity, which has been reported to hydrolyze PAF-like lipids to lysophosphatidyl-cholines. Preincubation of plasma LDL with diisopropyl fluorophosphate dose-dependently inhibited PAF-acetylhydrolase activity, resulting in accumulation of the PAF-like lipids when the LDL was oxidized with Cu2+. As well as PAF and lysophosphatidylcholines, several PAF-like lipids were found to inhibit [3H]thymidine incorporation ...Continue Reading

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Citations

Aug 30, 2001·Experimental Gerontology·G Spiteller
Jun 29, 2007·The Journal of Biological Chemistry·Rui ChenThomas M McIntyre
Aug 19, 2009·Antioxidants & Redox Signaling·Valery N BochkovJohannes Stöckl
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Sep 24, 2013·PloS One·Witigo von SchönfelsClemens Schafmayer
Mar 14, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Constantina NasopoulouIoannis Zabetakis
Aug 15, 2006·Biochimica Et Biophysica Acta·Gilbert O FruhwirthAlbin Hermetter
Jul 25, 2006·Life Sciences·Alexandros B TsouprasConstantinos A Demopoulos
May 25, 2015·Free Radical Biology & Medicine·Calivarathan LatchoumycandaneThomas M McIntyre
Mar 8, 2011·Experimental Biology and Medicine·Claudia PennaGiuseppe Alloatti
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