PMID: 9165297Jan 1, 1997Paper

Lipid peroxidation in photodynamically stressed mammalian cells: use of cholesterol hydroperoxides as mechanistic reporters

Free Radical Biology & Medicine
P G GeigerA W Girotti

Abstract

Photodynamic action of merocyanine 540, an antileukemic sensitizing dye, on murine L1210 cells results in the formation of lipid hydroperoxides and loss of cell viability. High-performance liquid chromatography with mercury cathode electrochemical detection was used for determining lipid oxidation products, including the following cholesterol-derived hydroperoxides: 5 alpha-OOH, 6 alpha-OOH, 6 beta-OOH, and unresolved 7 alpha, 7 beta-OOH. Among these species, 5 alpha-, 6 alpha-, and 6 beta-OOH (singlet oxygen adducts) were predominant in the early stages of photooxidation, whereas 7 alpha- and 7 beta-OOH (products of free radical reactions) became so after prolonged irradiation or during dark incubation after exposure to a light dose. These mechanistic changes were studied in a unique way by monitoring shifts in the peroxide ratio, i.e., 7-OOH/5 alpha-OOH, or 7-OOH/6-OOH. When cells (10(7)/ml) were exposed to a visible light fluence of 0.6 J/cm2 in the presence of 10 microM merocyanine 540, 7-OOH/5 alpha-OOH increased by approximately 100% after 2 h of dark incubation at 37 degrees C. The increase was much larger (approximately 250%) when cells were photooxidized after treatment with 1 microM ferric-8-hydroxyquinoline, a lipoph...Continue Reading

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Citations

May 24, 2003·Biochemical and Biophysical Research Communications·Yasukazu YoshidaEtsuo Niki
Mar 14, 2003·Free Radical Biology & Medicine·Adam WrightMichael J Davies
Oct 31, 2001·Journal of Photochemistry and Photobiology. B, Biology·A W Girotti
Mar 18, 2004·Antioxidants & Redox Signaling·Albert W Girotti, Tamas Kriska
Mar 12, 2008·Antioxidants & Redox Signaling·Michael J DaviesMartin D Rees
Jan 18, 2011·Free Radical Biology & Medicine·Agnieszka BroniecTadeusz Sarna
Jun 9, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·E AlarcónJ C Scaiano
Mar 19, 2011·Biology of Reproduction·Jos F BrouwersBart M Gadella
Dec 14, 2006·Free Radical Research·Dalal A Al-MutairiLudmil T Benov
Mar 22, 2000·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·T W Stief, J Fareed
Dec 5, 2017·PloS One·Malak ChararaLudmil Benov
Jul 3, 2018·Photochemistry and Photobiology·Albert W Girotti, Witold Korytowski
Dec 17, 2009·Lasers in Surgery and Medicine·Robert F JacksonRyan J Maloney
Jan 5, 2000·Physiological Reviews·G J Schroepfer
Dec 26, 2001·Chemical Reviews·H Ali, J E van Lier
Mar 28, 2002·Journal of the American Chemical Society·Nicole Haralampus-GrynaviskiJohn D Simon
Aug 17, 2010·Free Radical Biology & Medicine·Aldwin Suryo RahmantoMichael J Davies

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