Lipid peroxidation in the postnatal rat brain. Formation of 4-hydroxynonenal in the supraventricular corpus callosum of postnatal rats

Experimental Brain Research
Q CaoB Halliwell

Abstract

Lipid peroxidation is known to be associated with many neurodegenerative diseases and with traumatic brain injury, but its occurrence in the normal developing brain has not been reported. The present study was carried out using a specific antibody that recognises proteins modified by the end-product of lipid peroxide decomposition, 4-hydroxynonenal (HNE), to evaluate evaluate possible lipid peroxidation products in the brains of developing rats by immunocytochemistry and electron microscopy. Moderately dense labelling was observed in the supraventricular corpus callosum in the 7- and 8-day-old rats, whilst very dense labelling was observed in the same region, in the 9- and 10-day-old rats. Very little immunoreactivity was observed at 14 days, and no staining was observed in the corpus callosum in adult rats. HNE staining was not observed in neuronal cell bodies that give rise to callosal axons in the overlying cerebral cortex. Electron microscopy showed dense HNE staining on the basal laminae of blood vessels and on the plasma membranes of unmyelinated axons. Large numbers of rounded cells with features of oligodendrocyte precursor cells were labelled by Perl's stain in the supraventricular corpus callosum at postnatal day 7 an...Continue Reading

Citations

May 18, 2016·Oxidative Medicine and Cellular Longevity·Jiří WilhelmLuděk Vajner
Sep 8, 2006·Journal of Neuropathology and Experimental Neurology·Robin L HaynesHannah C Kinney
Sep 30, 2004·Journal of Neuropathology and Experimental Neurology·Rebecca D FolkerthHannah C Kinney

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