PMID: 9554830Apr 29, 1998Paper

Lipid peroxidation, tissue necrosis, and metabolic and mechanical recovery of isolated reperfused rat heart as a function of increasing ischemia

Free Radical Research
B TavazziG Lazzarino

Abstract

Isolated Langendorff-perfused rat hearts, after 30 min of preperfusion, were submitted to increasing times of global normothermic ischemia (1, 2, 5, 10, 20 and 30 min) or to the same times of ischemia followed by 30 min of reperfusion. Analysis of malondialdehyde, ascorbic acid, oxypurines, nucleosides, nicotinic coenzymes and high-energy phosphates was carried out by HPLC on neutralized perchloric acid extracts of freeze-clamped tissues. In addition, maximum rate of intraventricular pressure development and cardiac output of malondialdehyde, lactate dehydrogenase, oxypurines and nucleosides were monitored during both preperfusion and reperfusion. Besides decreasing energy metabolites and nicotinic coenzyme pool, prolonged ischemia produced oxidation of significant amounts of hypoxanthine and xanthine to uric acid and generation of detectable levels of malondialdehyde (0.002 micromol/g dry weight). After oxygen and substrate readmission, tissue and perfusate malondialdehyde increased only if previous ischemia was longer than 5 min, while lactate dehydrogenase was detected in perfusate of reperfused hearts following 10, 20, and 30 min of ischemia. Highest values of tissue malondialdehyde and total malondialdehyde output were rec...Continue Reading

References

Oct 1, 1976·The American Journal of Physiology·R NúñezF López-Soriano
Nov 1, 1979·The American Journal of Physiology·R J WeigelC L Prosser
Oct 1, 1992·Free Radical Biology & Medicine·T ParaidathathuJ P Kehrer
Sep 1, 1992·Cardiovascular Research·L H Opie
Feb 1, 1992·Journal of Molecular and Cellular Cardiology·P A UusimaaI E Hassinen
Feb 1, 1992·Cardiovascular Research·D J Hearse, R Bolli
Jun 1, 1991·The Journal of Clinical Investigation·G AmbrosioM Chiariello
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J L ZweierM L Weisfeldt
Dec 1, 1988·Journal of Molecular and Cellular Cardiology·V V KupriyanovV A Saks
Aug 1, 1988·The American Journal of Physiology·J A HoerterM Guéron
Aug 1, 1987·Journal of Molecular and Cellular Cardiology·V V KupriyanovV A Saks
Apr 28, 1994·Biochimica Et Biophysica Acta·D LautierG G Poirier
Jan 1, 1993·Free Radical Research Communications·J M Gutteridge

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Citations

Jan 7, 2004·Analytical Biochemistry·Giuseppe LazzarinoBarbara Tavazzi
Aug 30, 2002·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·F CarlucciA Tabucchi
Dec 31, 2002·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·F CarlucciE Marinello
Jun 24, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·B BiagioliF Carlucci
Nov 9, 2011·PM & R : the Journal of Injury, Function, and Rehabilitation·Stefano SignorettiRoberto Vagnozzi
Aug 20, 2011·Journal of Photochemistry and Photobiology. B, Biology·Victor MonichAnna Bavrina
Aug 29, 2001·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·L CristoforiG Lazzarino
Mar 12, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Yasmin E LoperaSusana Mosca
Apr 30, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Magomed KhaidakovJawahar L Mehta
Feb 3, 2007·Journal of Applied Physiology·Ah-Mee Park, Yuichiro J Suzuki

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