Lipid phosphate phosphatases form homo- and hetero-oligomers: catalytic competency, subcellular distribution and function

The Biochemical Journal
Jaclyn LongSusan Pyne

Abstract

Lipid phosphate phosphatases (LPP1-LPP3) have been topographically modelled as monomers (molecular mass of 31-36 kDa) composed of six transmembrane domains and with the catalytic site facing the extracellular side of the plasma membrane or the luminal side of intracellular membranes. The catalytic motif has three conserved domains, termed C1, C2 and C3. The C1 domain may be involved in substrate recognition, whereas C2 and C3 domains appear to participate in the catalytic dephosphorylation of the substrate. We have obtained three lines of evidence to demonstrate that LPPs exist as functional oligomers. First, we have used recombinant expression and immunoprecipitation analysis to demonstrate that LPP1, LPP2 and LPP3 form both homo- and hetero-oligomers. Secondly, large LPP oligomeric complexes that are catalytically active were isolated using gel-exclusion chromatography. Thirdly, we demonstrate that catalytically deficient guinea-pig FLAG-tagged H223L LPP1 mutant can form an oligomer with wild-type LPP1 and that wild-type LPP1 activity is preserved in the oligomer. These findings suggest that, in an oligomeric arrangement, the catalytic site of the wild-type LPP can function independently of the catalytic site of the mutant LP...Continue Reading

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Citations

Jun 30, 2012·Chemical Reviews·Bernard P C KokDavid N Brindley
Mar 3, 2009·The Journal of Biological Chemistry·Nadine HagenGerhild van Echten-Deckert
Aug 26, 2009·Molecular and Cellular Biology·Guang-Hui LiuLarry Gerace
May 17, 2011·Molecular Cancer·Ishita ChatterjeeKishore K Wary
Dec 11, 2008·Journal of Lipid Research·David N Brindley, Carlos Pilquil
Jul 28, 2012·Biochimica Et Biophysica Acta·H RenS S Smyth
Jun 28, 2012·Biochimica Et Biophysica Acta·Ana OliveraRichard L Proia
Aug 26, 2010·The Biochemical Journal·Guang-Hui LiuThurl E Harris
Mar 31, 2015·Journal of Lipid Research·Xiaoyun TangDavid N Brindley
Jul 13, 2016·FEBS Letters·Hayley E Bullen, Dominique Soldati-Favre
Mar 21, 2009·Current Opinion in Endocrinology, Diabetes, and Obesity

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