Lipid profiling of the soybean pathogen Phytophthora sojae using Fatty Acid Methyl Esters (FAMEs)

Fungal Biology
Lina Fayez YousefRichard P Dick

Abstract

Phytophthora sojae is a destructive soilborne pathogen of soybean, but currently there is no rapid or commercially available testing for its infestation level in soil. For growers, such information would greatly improve their ability to make management decisions to minimize disease damage to soybean crops. Fatty acid profiling of P. sojae holds potential for determining the prevalence of this pathogen in soil. In this study, the Fatty Acid Methyl Ester (FAME) profile of P. sojae was determined in pure culture, and the profile was subsequently evaluated for its potential use in detecting the pathogen in soil. The predominant fatty acids in the FAME profile of P. sojae are the unsaturated 18C fatty acids (18:1ω9 and 18:2ω6) followed by the saturated and unsaturated 16C fatty acids (16:0 and 16:1ω7). FAME analysis of P. sojae zoospores showed two additional long-chain saturated fatty acids (20:0 and 22:0) that were not detected in the mycelium of this organism. Addition of a known number of zoospores of P. sojae to soil demonstrated that fatty acids such as 18:1ω9, 18:2ω6, 20:1ω9, 20:4ω6, and 22:1ω9 could be detected and quantified against the background levels of fatty acids present in soil. These results show the potential for u...Continue Reading

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Citations

Mar 20, 2015·Journal of Agricultural and Food Chemistry·Shida MiaoLu-Kwang Ju
Oct 11, 2017·Molecular Plant Pathology·Sander Y A RodenburgFrancine Govers
May 16, 2017·Annual Review of Microbiology·Howard S Judelson
Nov 9, 2020·International Journal of Biological Macromolecules·Xiaoqing HuangMaozhi Ren
Jul 8, 2020·Journal of Agricultural and Food Chemistry·Dong LiuZhimou Gao

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