LipiD-QuanT: a novel method to quantify lipid accumulation in live cells.

Journal of Lipid Research
Hilal VarinliPascal Vallotton

Abstract

Lipid droplets (LDs) are the main storage organelles for triglycerides. Elucidation of lipid accumulation mechanisms and metabolism are essential to understand obesity and associated diseases. Adipogenesis has been well studied in murine 3T3-L1 and human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell lines. However, most techniques for measuring LD accumulation are either not quantitative or can be destructive to samples. Here, we describe a novel, label-free LD quantification technique (LipiD-QuanT) to monitor lipid dynamics based on automated image analysis of phase contrast microscopy images acquired during in vitro human adipogenesis. We have applied LipiD-QuanT to measure LD accumulation during differentiation of SGBS cells. We demonstrate that LipiD-QuanT is a robust, nondestructive, time- and cost-effective method compared with other triglyceride accumulation assays based on enzymatic digest or lipophilic staining. Further, we applied LipiD-QuanT to measure the effect of four potential pro- or antiobesogenic substances: DHA, rosiglitazone, elevated levels of D-glucose, and zinc oxide nanoparticles. Our results revealed that 2 µmol/l rosiglitazone treatment during adipogenesis reduced lipid production and caused ...Continue Reading

References

Apr 10, 1999·Postgraduate Medical Journal·A H ZargarA I Wani
Mar 13, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·M WabitschH Hauner
Aug 23, 2001·Current Biology : CB·D A Brown
Nov 15, 2002·Histochemistry and Cell Biology·Satoshi Fukumoto, Toyoshi Fujimoto
Jul 4, 2003·Current Opinion in Lipidology·Guenter HaemmerleRudolf Zechner
Sep 10, 2004·The New England Journal of Medicine·Hannele Yki-Järvinen
Nov 13, 2004·The Journal of Biological Chemistry·Ying LinPhilipp E Scherer
Mar 1, 2005·Biochimie·C LondosA R Kimmel
Mar 3, 2005·Journal of Lipid Research·Horst RobenekDavid Troyer
Nov 29, 2005·Best Practice & Research. Clinical Endocrinology & Metabolism·Bruno Fève
Dec 13, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·James KacmarFriedrich Srienc
Feb 1, 2006·International Journal of Obesity : Journal of the International Association for the Study of Obesity·M GarauletS Zamora
Nov 23, 2006·The Journal of Nutrition·Hye-Kyeong KimClifton A Baile
Sep 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Thomas HellererAnnika Enejder
Oct 11, 2007·Chemical Reviews·Aurore Loudet, Kevin Burgess
Jan 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Paola AguiariRosario Rizzuto
Sep 10, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·R E WeaverA J Balmforth
May 21, 2009·The British Journal of Nutrition·Michelle MicallefManohar Garg
May 23, 2009·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·J D Buckley, P R C Howe
Sep 25, 2009·Journal of Lipid Research·Thuc T LeJi-Xin Cheng
Oct 20, 2009·Biochimica Et Biophysica Acta·Juan Carlos MoleroKen Walder
Jan 1, 2008·Obesity Facts·Pamela Fischer-PosovszkyHans E Tornqvist
Sep 25, 2010·Experimental Biology and Medicine·Sylvia P PoulosGary J Hausman
Jan 14, 2011·International Journal of Pediatric Obesity : IJPO : an Official Journal of the International Association for the Study of Obesity·T BurrowsM L Garg
Feb 11, 2011·Journal of Atherosclerosis and Thrombosis·Emiko SaitoYohei Kitamura
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Toyoshi Fujimoto, Robert G Parton
Apr 20, 2011·Circulation·Michael MillerUNKNOWN Council on the Kidney in Cardiovascular Disease
Aug 24, 2011·Journal of Electron Microscopy·Michitaka SuzukiToyoshi Fujimoto
Nov 11, 2011·International Journal of Molecular Sciences·Pablo CalzadillaLiliana N Guerra
Feb 9, 2012·The Journal of Biological Chemistry·Margret PaarHeimo Wolinski
Jun 1, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Judith T CirulisGregory M Ross
Aug 3, 2012·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Emma H AllottGraham P Pidgeon
Aug 18, 2012·Journal of Lipid Research·Alexandra RobciucJuha M Holopainen

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Citations

Feb 18, 2017·International Journal of Molecular Sciences·Veronica GhiniAlessandra Bordoni
Oct 26, 2018·The Analyst·Anushka GuptaAaron M Streets
Jul 11, 2020·Organic & Biomolecular Chemistry·Palash JanaSriram Kanvah
Jan 9, 2021·Cell Death & Disease·Natalia P AlzaGabriela A Salvador
Jun 14, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Arpana Singh, Prosenjit Sen

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