Lipid recovery from a vegetable oil emulsion using microbial enrichment cultures

Biotechnology for Biofuels
Jelmer TamisRobbert Kleerebezem

Abstract

Many waste streams have a relatively high vegetable oil content, which is a potential resource that should be recovered. Microbial storage compound production for the recovery of lipids from lipid-water emulsions with open (unsterilized) microbial cultures was investigated in a sequencing batch reactor using a diluted vegetable oil emulsion as model substrate. After feeding, triacylglycerides (TAG) were accumulated intracellular by the microbial enrichment culture and subsequently used for growth in the remainder of the sequencing batch cycle. Roughly 50% of the added TAG could be recovered as intracellular lipids in this culture. The maximum lipid storage capacity of the enrichment culture was 54% on volatile suspended solids (VSS) mass basis in a separate fed-batch accumulation experiment. The microbial community was dominated by a lipolytic fungus, Trichosporon gracile, that was responsible for intracellular lipid accumulation but also a significant fraction of lipolytic and long chain fatty-acid-utilizing bacteria was present. Herewith, we demonstrate an effective strategy for enrichment of a microbial community that can accumulate significant amounts of lipids from wastewaters without the need for sterilization of substrat...Continue Reading

References

Jan 1, 1978·Annual Review of Microbiology·E Juni
Jun 1, 1987·Lipids·V K Babayan
Jun 29, 1999·Current Opinion in Microbiology·G Muyzer
Dec 6, 2002·Applied Microbiology and Biotechnology·H M Alvarez, A Steinbüchel
Sep 19, 2003·Bioprocess and Biosystems Engineering·M A M ReisM C M Van Loosdrecht
Jun 9, 2004·Applied and Environmental Microbiology·Matthias ArenskötterAlexander Steinbüchel
Feb 24, 2006·Journal of Industrial Microbiology & Biotechnology·K B Chipasa, K Medrzycka
May 19, 2007·Current Opinion in Biotechnology·Robbert Kleerebezem, Mark C M van Loosdrecht
Jun 10, 2008·Water Environment Research : a Research Publication of the Water Environment Federation·Hsin-ying LiuFrank J Loge
Feb 6, 2009·Biomacromolecules·Katja JohnsonMark C M van Loosdrecht
Feb 17, 2010·Journal of Biotechnology·Katja JohnsonMark C M van Loosdrecht
Nov 12, 2010·Water Research·Yang JiangMark C M van Loosdrecht
Dec 31, 2010·Trends in Biotechnology·Matthew T AglerLargus T Angenent
Apr 2, 2011·Biotechnology and Bioengineering·Yang JiangMark C M van Loosdrecht
Jun 16, 2011·Applied Microbiology and Biotechnology·Helena Moralejo-GárateMark C M van Loosdrecht
Aug 28, 2012·Water Research·Yang JiangRobbert Kleerebezem
May 21, 2013·Progress in Lipid Research·Ming-Hua Liang, Jian-Guo Jiang
Dec 24, 2013·New Biotechnology·Simon AnterrieuAlan Werker
Feb 4, 2014·Current Opinion in Biotechnology·Catherine M SpiritoLargus T Angenent
Mar 7, 2014·Biotechnology for Biofuels·Fabio SantamauroChristopher J Chuck
Apr 29, 2014·Current Opinion in Biotechnology·Emilie El MullerPaul Wilmes
May 8, 2014·International Journal of Biological Macromolecules·Leonie MarangRobbert Kleerebezem
Dec 3, 2014·Current Opinion in Biotechnology·Peter R MooijRobbert Kleerebezem

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Citations

Aug 1, 2015·PLoS Pathogens·Marçal MarinéGustavo Henrique Goldman
Jun 29, 2017·International Journal of Qualitative Studies on Health and Well-being·Lindsay FavottoColleen Davison
Nov 6, 2020·The Science of the Total Environment·Lucía ArgizAnuska Mosquera-Corral
Apr 11, 2021·Biotechnology Advances·Fabrizio Di Caprio

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Methods Mentioned

BETA
electrophoresis
PCR

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