Lipid-specific membrane activity of human beta-defensin-3

Biochemistry
Arne BöhlingThomas Gutsmann

Abstract

Defensins represent a major component of innate host defense against bacteria, fungi, and enveloped viruses. One potent defensin found, e.g., in epithelia, is the polycationic human beta-defensin-3 (hBD3). We investigated the role of the lipid matrix composition, and in particular the presence of negatively charged lipopolysaccharides (LPS) from sensitive (Escherichia coli, Salmonella enterica serovar Minnesota) or resistant (Proteus mirabilis) Gram-negative bacteria or of the zwitterionic phospholipids of human cells, in determining the action of polycationic hBD3 on the different membranes, and related to their biological activity. The main focus was directed on data derived from electrical measurements on a reconstitution system of the OM as a planar asymmetric bilayer composed on one side of LPS and on the other of a phospholipid mixture. Our results demonstrate that the antimicrobial activity and the absence of cytotoxicity can be explained by the lipid-specificity of the peptide. A clear correlation between these aspects of the biological activity of hBD3 and its interaction with lipid matrices could be found. In particular, hBD3 could only induce lesions in those membranes resembling the lipid composition of the OM of se...Continue Reading

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Citations

Jun 7, 2011·Journal of Industrial Microbiology & Biotechnology·Pengwei Zhao, Guifang Cao
Jul 27, 2007·Natural Product Reports·Sophie DuquesneSylvie Rebuffat
Jan 19, 2008·Clinical Microbiology Reviews·S M JacobsenM E Shirtliff
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May 16, 2014·Pharmaceuticals·Guangshun Wang
Feb 7, 2016·Biochimica Et Biophysica Acta·Thomas Gutsmann
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Mar 19, 2015·PloS One·Himanshu Sharma, Ramakrishnan Nagaraj
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Mar 17, 2020·Biochimica Et Biophysica Acta. Biomembranes·Christian NehlsThomas Gutsmann
Jun 2, 2009·Biochemistry·Karthik Balakrishna ChandrababuDaiwen Yang

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