Lipid specificity of surfactant protein B studied by time-of-flight secondary ion mass spectrometry.

Biophysical Journal
Daniel BreitensteinH J Galla

Abstract

One of the key functions of mammalian pulmonary surfactant is the reduction of surface tension to minimal values. To fulfill this function it is expected to become enriched in dipalmitoylphosphatidylcholine either on its way from the alveolar type II pneumocytes to the air/water interface of the lung or within the surface film during compression and expansion of the alveoli during the breathing cycle. One protein that may play a major role in this enrichment process is the surfactant protein B. The aim of this study was to identify the lipidic interaction partner of this protein. Time-of-flight secondary ion mass spectrometry was used to analyze the lateral distribution of the components in two SP-B-containing model systems. Either native or partly isotopically labeled lipids were analyzed. The results of both setups give strong indications that, at least under the specific conditions of the chosen model systems (e.g., concerning pH and lipid composition), the lipid interacting with surfactant protein B is not phosphatidylglycerol as generally accepted, but dipalmitoylphosphatidylcholine instead.

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Citations

Sep 16, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Matthew J BakerJohn C Vickerman
Oct 23, 2009·Journal of the Royal Society, Interface·Rakesh Kumar HarishchandraHans-Joachim Galla
Dec 18, 2008·Annual Review of Biophysics·Steven G BoxerPeter K Weber
Dec 7, 2007·Biophysical Journal·Jesús Pérez-Gil
Nov 30, 2010·Biochimica Et Biophysica Acta·Eleonora KeatingNils O Petersen
Mar 15, 2015·Biochimica Et Biophysica Acta·Fábio FernandesLuís M S Loura
Nov 3, 2009·Biochimica Et Biophysica Acta·Mohammed Saleem, Hans-Joachim Galla
Mar 28, 2007·Journal of Mass Spectrometry : JMS
Dec 21, 2007·The Journal of Biological Chemistry·Mohammed SaleemHans-Joachim Galla
Jul 4, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Rainer KassenböhmerHeinrich F Arlinghaus
Mar 29, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Lin WangRichard Mendelsohn

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