PMID: 7026237Aug 1, 1981Paper

Lipid-synthesis-dependent biosynthesis (or assembly) of major outer-membrane proteins of Escherichia coli

European Journal of Biochemistry
C Bocquet-PagesA Lazdunski

Abstract

Upon inhibition of fatty acid synthesis in the presence of cerulenin, the uptake of 5' AMP and of other nutrients using similar pore systems can be inhibited as much as 70%. The same effect was observed upon inhibition of phospholipid synthesis after glycerol deprivation in a mutant strain defective in sn-glycerol-3-phosphate acyltransferase. Resumption of both fatty acid synthesis and phospholipid synthesis restores a normal uptake of 5' AMP. The protein composition of the outer membranes, analyzed after pulse radiolabelling by [35S]methionine, was mainly altered in OmpF and OmpC proteins. These proteins are the main porins used by most nutrients like 5' AMP. Whereas the synthesis or the assembly of OmpF protein seems to be more inhibited that that of OmpC protein after inhibition of fatty acid synthesis, the reverse case was observed after inhibition of phospholipid synthesis. No protein produced during inhibition of fatty-acid or phospholipid synthesis is reincorporated into the outer membrane after resumption of these syntheses. These results are discussed with regard to the biosynthesis and assembly of these proteins.

References

Feb 25, 1979·Journal of Molecular Biology·A LazdunskiC Lazdunski
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P K Chattopadhyay, H C Wu
Feb 1, 1978·Journal of Bacteriology·M Leduc, M Schaechter
Jan 1, 1978·Annual Review of Biochemistry·J M DiRienzoM Inouye
Sep 23, 1976·Molecular & General Genetics : MGG·W van AlphenW Berendsen
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
May 1, 1973·Antimicrobial Agents and Chemotherapy·I GoldbergK Bloch

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Citations

Feb 1, 1982·European Journal of Biochemistry·C PagesA Lazdunski
Jun 10, 2000·European Journal of Biochemistry·C JansenH de Cock
Jan 14, 2011·Bioscience, Biotechnology, and Biochemistry·Kimiko WatanabeKoichiro Isoi
May 15, 1992·European Journal of Biochemistry·D FourelJ M Pagès
Nov 7, 1998·Biochimica Et Biophysica Acta·W Dowhan
Dec 1, 2011·EcoSal Plus·Juliana C Malinverni, Thomas J Silhavy
Jan 1, 1983·Journal of Bacteriology·R K Tweten, J J Iandolo
Nov 3, 1989·Biochimica Et Biophysica Acta·H de CockJ Tommassen

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