Lipid-Targeting Peptide Probes for Extracellular Vesicles.

Journal of Cellular Physiology
Aaron D Flynn, Hang Yin

Abstract

Extracellular vesicles released from cells are under intense investigation for their roles in cell-cell communication and cancer progression. However, individual vesicles have been difficult to probe as their small size renders them invisible by conventional light microscopy. However, as a consequence of their small size these vesicles possess highly curved lipid membranes that offer an unconventional target for curvature-sensing probes. In this article, we present a strategy for using peptide-based biosensors to detect highly curved membranes and the negatively charged membrane lipid phosphatidylserine, we delineate several assays used to validate curvature- and lipid-targeting mechanisms, and we explore potential applications in probing extracellular vesicles released from sources such as apoptotic cells, cancer cells, or activated platelets. J. Cell. Physiol. 231: 2327-2332, 2016. © 2016 Wiley Periodicals, Inc.

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Citations

Oct 14, 2017·Expert Review of Proteomics·Barbara W SódarEdit I Buzás
May 28, 2020·Nature Reviews. Molecular Cell Biology·Killian O'BrienXandra O Breakefield
May 23, 2017·Frontiers in Physiology·Carina HromadaWolfgang Holnthoner
May 5, 2020·Journal of Extracellular Vesicles·Alessandro GoriMarina Cretich

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