Lipid Topography in Schistosoma mansoni Cryosections, Revealed by Microembedding and High-Resolution Atmospheric-Pressure Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Imaging

Analytical Chemistry
Patrik KadeschBernhard Spengler

Abstract

Schistosomes are parasitic platyhelminthes that cause schistosomiasis, which is a life-threatening infectious disease for humans in the tropics and subtropics worldwide. Within the human host, female and male schistosomes develop and pair as a prerequisite for egg production. Part of the eggs get lodged in organs such as the gut, spleen, and liver, where they cause severe inflammatory processes, including liver fibrosis, which is one of the most serious pathological symptoms. High-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization (AP-SMALDI) mass spectrometry imaging (MSI) has been used as a powerful tool to investigate adult schistosomes at the topographic molecular level. An MSI-compatible protocol was developed, covering critical sample preparation steps and focusing on obtaining artifact-free, longitudinal cryosections. Planar, consecutive sections were prepared from ∼400 μm thick S. mansoni worm couples, comparing several microembedding approaches. High-resolution MSI at both, 10 and 5 μm lateral resolution unraveled anatomical structures and differential abundances of glycerophospholipids and saccharides in females and males. In addition, glycerophospholipids occurred differen...Continue Reading

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Jun 24, 2020·Journal of Separation Science·Na LiHuwei Liu
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