Lipidomic markers of sperm cryotolerance in cattle

Scientific Reports
Holly C EvansErdogan Memili

Abstract

The objective of the current study was to determine the fatty acid composition of sperm from Holstein bulls with different freezability (Good and Poor; n = 12). Fatty acids were extracted from frozen sperm in 1:2 (v/v) chloroform-methanol solvent, fractionated into neutral and polar fractions, and composition determined by gas chromatography-mass spectrometry. Thirty-four fatty acids were quantified and their concentrations and percentages within each lipid fraction were calculated. Overall, saturated fatty acids (SFA) were predominant, accounting for 71 to 80% of fatty acids in neutral and polar lipid factions. There were marked differences in fatty acid composition between the lipid fractions (P < 0.001). The branched chain fatty acid (BCFA) concentration (15 to 18 µg) was almost twice as much as polyunsaturated fatty acids (PUFA) concentration found in the polar lipid fraction (8 to 9 µg; P < 0.001). Sperm with different freezability phenotypes only had a few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (P ≤ 0.011). These results are significant because they reveal key understandings of fatty acid composition of sperm membrane and lay a foundation for the manipulation of membrane integrity, fluidity, and s...Continue Reading

References

May 24, 1973·Biochimica Et Biophysica Acta·A PoulosI G White
May 1, 1980·Quarterly Reviews of Biophysics·J N IsraelachviliR G Horn
May 23, 1998·Molecular Human Reproduction·A A ZalataF H Comhaire
Jun 14, 2000·Animal Reproduction Science·P F Watson
Aug 10, 2000·The Journal of Biological Chemistry·E DuplusC Forest
Sep 28, 2000·Human Reproduction·M N GiraudG Grizard
Aug 9, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Yilmaz AksoyAsuman Ozkan
Sep 15, 2007·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Ali Khosrowbeygi, Nosratollah Zarghami
May 12, 2010·Reproduction in Domestic Animals = Zuchthygiene·B Macías GarcíaF J Peña
Nov 20, 2012·Molecular & Cellular Proteomics : MCP·Alexandra AmaralRafael Oliva
May 8, 2015·Andrology·V EsmaeiliA R Alizadeh
Mar 10, 2016·Asian-Australasian Journal of Animal Sciences·Len Van TranAmrish Kumar Tyagi
Dec 12, 2017·Developmental Biology·Laure-Anne TeuwenPeter Carmeliet
Oct 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carla C C R de Carvalho, Maria José Caramujo
Jun 30, 2019·Metabolites·Abdul-Hamid EmwasDavid S Wishart
Sep 20, 2019·BMC Genomics·E B MenezesE Memili
Sep 26, 2019·Frontiers in Veterinary Science·Muhammet Rasit UgurErdogan Memili
Jan 30, 2020·Frontiers in Cell and Developmental Biology·Muhammet Rasit UgurErdogan Memili

❮ Previous
Next ❯

Citations

May 4, 2021·Frontiers in Veterinary Science·Holly C EvansErdogan Memili
Aug 28, 2021·International Journal of Molecular Sciences·Shuwen ShanBertram Brenig

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear magnetic resonance
NMR

Software Mentioned

SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.