Lipidomics as an important key for the identification of beer-spoilage bacteria

Letters in Applied Microbiology
T ŘezankaK Sigler

Abstract

Electrospray ionization-tandem mass spectrometry (ESI-MS/MS) was used for characterizing intact plasmalogen phospholipid molecules in beer-spoilage bacteria. Identification of intact plasmalogens was carried out using collision-induced dissociation and the presence of suitable marker molecular species, both qualitative and quantitative, was determined in samples containing the anaerobic bacteria Megasphaera and Pectinatus. Using selected ion monitoring (SIM), this method had a limit of detection at 1 pg for the standard, i.e. 1-(1Z-octadecenyl)-2-oleoyl-sn-glycero-3-phosphoethanolamine and be linear in the range of four orders of magnitude from 2 pg to 20 ng. This technique was applied to intact plasmalogen extracts from the samples of contaminated and uncontaminated beer without derivatization and resulted in the identification of contamination of beer by Megasphaera and Pectinatus bacteria. The limit of detection was about 830 cells of anaerobic bacteria, i.e. bacteria containing natural cyclopropane plasmalogenes (c-p-19:0/15:0), which is the majority plasmalogen located in both Megasphaera and Pectinatus. The SIM ESI-MS method has been shown to be useful for the analysis of low concentration of plasmalogens in all biologica...Continue Reading

References

Nov 1, 1996·International Journal of Food Microbiology·L Jespersen, M Jakobsen
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Nov 19, 2003·International Journal of Food Microbiology·Kanta Sakamoto, Wil N Konings
Sep 13, 2008·Journal of Applied Microbiology·D G WeberG Antranikian
Jul 20, 2010·Progress in Lipid Research·Howard Goldfine
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Nov 8, 2011·International Journal of Systematic and Evolutionary Microbiology·Wen-wu ZhangXu-fen Zhu
Apr 23, 2013·International Journal of Systematic and Evolutionary Microbiology·Jane M CaldwellFred Breidt

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Citations

Feb 16, 2018·Lipids·Tomáš ŘezankaKarel Sigler
Apr 21, 2020·Metabolomics : Official Journal of the Metabolomic Society·Jeremy P KoelmelJohn A Bowden
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Ann-Britt Schäfer, Michaela Wenzel
Jun 3, 2021·Microorganisms·Lucia GharwalováTomáš Řezanka
Jun 21, 2021·Progress in Lipid Research·Milada VítováTomáš Řezanka

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