Lipids by Yarrowia lipolytica Strains Cultivated on Glucose in Batch Cultures

Microorganisms
E CarsanbaH Erten

Abstract

Oleaginous microorganisms, such as Yarrowia lipolytica, accumulate lipids that can have interesting applications in food biotechnology or the synthesis of biodiesel. Y. lipolytica yeast can have many advantages such as wide substrate range usage and robustness to extreme conditions, while under several culture conditions it can produce high lipid productivity. Based on this assumption, in this study, 12 different Yarrowia lipolytica strains were used to investigate microbial lipid production using a glucose-based medium under nitrogen-limited conditions in shake-flask cultivations. Twelve wild-type or mutant strains of Yarrowia lipolytica which were newly isolated or belonged to official culture collections were tested, and moderate lipid quantities (up to 1.30 g/L) were produced; in many instances, nitrogen limitation led to citric acid production in the medium. Lipids were mainly composed of C16 and C18 fatty acids. Most of the fatty acids of the microbial lipid were unsaturated and corresponded mainly to oleic, palmitic and linoleic acids. Linolenic acid (C18:3) was produced in significant quantities (between 10% and 20%, wt/wt of dry cell weight (DCW)) by strains H917 and Po1dL.

References

Sep 2, 2009·Progress in Lipid Research·Athanasios BeopoulosJean-Marc Nicaud
Apr 3, 2012·Bioresource Technology·Pierre FontanilleChristian Larroche
Mar 4, 2015·Metabolic Engineering·Kangjian QiaoGregory Stephanopoulos
Dec 26, 2015·Progress in Lipid Research·Rodrigo Ledesma-Amaro, Jean-Marc Nicaud
Sep 7, 2016·Biotechnology for Biofuels·Aleksandra M MirończukAdam Dobrowolski
Sep 23, 2016·Critical Reviews in Biotechnology·Ana Maria de Oliveira FincoCarlos Ricardo Soccol
Oct 16, 2016·Preparative Biochemistry & Biotechnology·Karla Silva Teixeira SouzaDisney Ribeiro Dias
Oct 21, 2016·Frontiers in Microbiology·Katrin OchsenreitherChristoph Syldatk
Nov 20, 2016·Biotechnology and Bioengineering·Dongming XieQuinn Zhu
Jun 24, 2017·Bioresource Technology·Lei QinDong Wei
Aug 13, 2017·Applied Microbiology and Biotechnology·Marianna DourouGeorge Aggelis
Nov 7, 2017·FEMS Yeast Research·Ema CavalloMaría Laura Foresti
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Nov 21, 2016·Engineering in Life Sciences·Sofia MainaApostolis Koutinas

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Methods Mentioned

BETA
fatty acid profile
PCA

Software Mentioned

PAST
SPSS

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