Lipids in blood-brain barrier models in vitro II: Influence of glial cells on lipid classes and lipid fatty acids

In Vitro Cellular & Developmental Biology. Animal
Stefanie D KrämerDavid J Begley

Abstract

Lipids of brain tissue and brain microvascular endothelial cells contain high proportions of long-chain polyunsaturated fatty acids (long PUFAs). The blood-brain barrier (BBB) is formed by the brain endothelial cells under the inductive influence of brain cells, especially perivascular glia, and coculture of endothelial cells and glial cells has been used to examine this induction. The objective of this study was to investigate whether C6 glioma cells are able to influence the lipid composition and shift the fatty acid (FA) patterns of the BBB model cell lines RBE4 and ECV304 toward the in vivo situation. Lipid classes of the three cell lines were analyzed by thin-layer chromatography and lipid FA patterns by high-performance liquid chromatography. Only ECV304 cells showed altered lipid composition in coculture with C6 cells. The fractions of triglycerides and cholesteryl esters (depending on the support filter) were about twice as high in coculture as when the cells were grown alone. Triglyceride fractions reached 13 to 15% of total lipids in coculture. The three cell lines showed an increase in the percentage of long PUFAs with respect to unsaturated FAs, mainly because of an increase in the percentages of arachidonic acid, a...Continue Reading

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Citations

Jan 4, 2012·Journal of Pharmaceutical Sciences·Pooja Naik, Luca Cucullo
Jun 14, 2005·Toxicon : Official Journal of the International Society on Toxinology·Luciana de Paula Le SueurMaria Alice da Cruz-Höfling
Sep 5, 2009·Investigational New Drugs·Umadevi SubramanianA J Vanisree
Apr 3, 2013·Endocrinology·Fiona ConnollyW Colin Duncan
Jul 30, 2005·Mass Spectrometry Reviews·Susumu Ando, Yasukazu Tanaka
Jun 26, 2020·Frontiers in Physiology·Hannah ChewAlfred N Fonteh
Dec 18, 2004·Alternatives to Laboratory Animals : ATLA·Pilar PrietoUNKNOWN European Centre for the Validation of Alternative Methods
Dec 4, 2003·Drug Discovery Today·Kuresh A YoudimN Joan Abbott

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