Lipids in bone: optimal conditions for tissue storage prior to lipid analyses

Calcified Tissue International
P Mularchuk, A L Boskey

Abstract

Optimal conditions for storage of calcified tissues prior to lipid analysis were determined by comparing the lipid composition (total lipid, total phospholipid, triglyceride, free fatty acid, cholesterol, and complexed acidic phospholipid) of mature rabbit diaphyseal bone stored 1, 2, and 5 months after animal sacrifice to that observed in the bones from which the lipids were immediately extracted at the time of sacrifice. Storage of lyophilized or nonlyophilized tissues at -70 degrees, or lyophilized tissues at -20 degrees, were the only methods that did not yield significant changes in bone lipid composition with time.

References

Mar 31, 1976·Calcified Tissue Research·A L Boskey, A S Posner
Dec 28, 1977·Calcified Tissue Research·J S EllingsonL R Larson
May 1, 1977·Calcified Tissue Research·A L Boskey, A S Posner
Mar 1, 1973·Archives of Oral Biology·R E ProutF C Tring
Jan 1, 1970·Calcified Tissue Research·T R Dirksen, G V Marinetti
Jan 1, 1970·Calcified Tissue Research·I M Shapiro
May 1, 1984·Calcified Tissue International·B D Boyan, N M Ritter
Jan 1, 1983·Metabolic Bone Disease & Related Research·A L Boskey, D M Timchak
Feb 1, 1980·Archives of Biochemistry and Biophysics·A L BoskeyD M Cordella

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Citations

Jan 1, 1996·Progress in Histochemistry and Cytochemistry·M Goldberg, A L Boskey
Mar 21, 2012·Journal of Mass Spectrometry : JMS·Sophie CersoyAlain Brunelle
Jun 30, 2015·Progress in Lipid Research·Alexandrine DuringPierre Hardouin
Jul 5, 2016·Physical Chemistry Chemical Physics : PCCP·Kamal H MroueAyyalusamy Ramamoorthy

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