Lipids of brush border membrane vesicles (BBMV) from Plutella xylostella resistant and susceptible to Cry1Ac delta-endotoxin of Bacillus thuringiensis

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
N S KumaraswamiH Hori

Abstract

Plutella xylostella (PX) that were 130000-fold more resistant to Cry1Ac were selected from the susceptible strain and maintained in the laboratory. The LC50 of the susceptible strain (PXS) was 0.38 microg toxin/g diet, whereas that of the resistant strain (PXR) was 4800 microg toxin/g diet. Brush border membrane vesicles (BBMV) were prepared from both PXS and PXR. In ligand blot analysis, Cry1Ac bound to a 120-kDa protein of BBMV; however, the intensity of the band was almost equal in both strains of insect. Hence, we analyzed the lipid components of BBMV from PXS and PXR. BBMV lipids were fractionated into non-polar lipid, phospholipid, neutral glycolipid and acidic glycolipid. Neutral glycolipid content was substantially lower in the BBMV of PXR than of PXS. The same trend was observed when lipids were extracted from whole midgut instead of BBMV. Thin layer chromatography of midgut neutral glycolipids revealed the presence of more than seven components. Among the midgut neutral glycolipids, a possible hexasaccharylceramide and a possible trisaccharylceramide of PXR were less than half the level found in PXS. The other lipid fractions in PXR and PXS were similar to each other.

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Citations

Dec 31, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jingquan LiuDongyu Liang
Mar 24, 2017·Journal of Economic Entomology·B PetersonJ Van den Berg
Mar 11, 2017·Analytical Biochemistry·Yun WangXian-Jin Liu
May 14, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Joel S Griffitts, Raffi V Aroian
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Yutao Xiao, Kongming Wu
Jun 5, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masahiro HiguchiHidetaka Hori
Jan 22, 2008·Insect Biochemistry and Molecular Biology·Simon W BaxterDavid G Heckel

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