PMID: 6967738Aug 11, 1980Paper

Lipids of plasma membranes from rat thymic lymphoid cells: deficiency of sphingomyelin

Biochimica Et Biophysica Acta
K KoizumiK Ota

Abstract

Plasma membranes were isolated from normal thymocytes of Wistar-King-A rats and from Moloney virus-induced rat thymic leukemias (RML11 and RML30 cells) using a simplified method developed by us. All the isolated plasma membranes were electron-microscopically pure and enriched in the specific activities of (Na+ + K+)-ATPase, Mg2+-ATPase and 5'-nucleotidase in comparison with those of the corresponding whole cell homogenates. These plasma membranes as well as the original cells were analyzed for phopholipid composition and contents of phospholipid, cholesterol and plasmalogen. There was no difference in the phospholipid composition among the three plasma membranes. However, all the plasma membranes were deficient in sphingomyelin, namely, 1.8% for the normal thymocytes, 2.2% for the RML11 cells and 1.9% for the RML30 cells as percentage of the total phospholipid phosphorus. The contents of phospholipid (mumol per mg protein), cholesterol (mumol per mg protein) and plasmalogen (mol% to phospholipid) of the plasma membranes from both lines of malignant cells were lower than those of the normal thymocyte membranes. The molar ratio of cholesterol to phospholipid of the malignant cell membranes was also lower than that of the normal m...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M PetitouM Inbar
Apr 16, 1976·Biochimica Et Biophysica Acta·M Shinitzky, M Inbar
Mar 29, 1973·Biochimica Et Biophysica Acta·W J van BlitterswijkC A Feltkamp
Jul 12, 1974·Biochimica Et Biophysica Acta·C T LadoulisT J Gill
Jun 21, 1973·Biochimica Et Biophysica Acta·G G Forstner, J R Wherrett
May 9, 1972·Biochimica Et Biophysica Acta·E FerberW Imm
Oct 2, 1967·Biochimica Et Biophysica Acta·G J Nelson
Jan 25, 1980·Biochimica Et Biophysica Acta·E FerberH U Weltzien

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Citations

Mar 21, 2006·International Journal of Radiation Biology·Heung Soon ParkMyung-Un Choi
Apr 15, 1992·European Journal of Biochemistry·W B MackinnonC E Mountford
Sep 1, 1983·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·B S FomenkoI G Akoev
Apr 30, 1993·Biochimica Et Biophysica Acta·S AndoT J Santoro

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