Lipids that determine detergent resistance of MDCK cell membrane fractions

Chemistry and Physics of Lipids
Marco M ManniFélix M Goñi

Abstract

A comparative lipidomic study has been performed of whole Madin-Darby canine kidney epithelial cells and of the detergent-resistant membrane fraction (DRM) obtained after treating the cells with the non-ionic detergent Triton X-100. The DRM were isolated following a standard procedure that is extensively used in cell biology studies. Significant differences were found in the lipid composition of the whole cells and of DRM. The latter were enriched in all the analyzed sphingolipid classes: sphingomyelins, ceramides and hexosylceramides. Diacylglycerols were also preferentially found in DRM. The detergent-resistant fraction was also enriched in saturated over unsaturated fatty acyl chains, and in sn-1 acyl chains containing 16 carbon atoms, over the longer and shorter ones. The glycerophospholipid species phosphatidylethanolamines and phosphatidylinositols, that were mainly unsaturated, did not show a preference for DRM. Phosphatidylcholines were an intermediate case: the saturated, but not the unsaturated species were found preferentially in DRM. The question remains on whether these DRM, recovered from detergent-membrane mixtures by floatation over a sucrose gradient, really correspond to membrane domains existing in the cell m...Continue Reading

References

Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Nov 13, 1987·Biochimica Et Biophysica Acta·M A UrbanejaA Alonso
Apr 1, 1980·Journal of Bioenergetics and Biomembranes·J I GurtubayJ M Macarulla
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sebastian SchuckKai Simons
Jul 6, 2005·Trends in Biochemical Sciences·Dov LichtenbergHeiko Heerklotz
Sep 15, 2007·Nature Protocols·Daniel Lingwood, Kai Simons
Dec 17, 2008·Quarterly Reviews of Biophysics·Heiko Heerklotz
Jan 8, 2013·Trends in Biochemical Sciences·Dov LichtenbergFélix M Goñi
Mar 19, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Maite Martínez-UñaJosé M Mato
Apr 24, 2013·The Journal of Biological Chemistry·Jessica F FriszMary L Kraft
May 8, 2013·The FEBS Journal·Matthias P Wymann, Kai Simons
Jul 23, 2013·Biophysical Journal·Dov LichtenbergFélix M Goñi
Sep 17, 2013·Advances in Nutrition·Alfred H MerrillEoin Fahy
Nov 7, 2014·Biochemistry·Kenneth D'SouzaRichard M Epand
Feb 11, 2015·Journal of Proteomics·Ibon Martínez-ArranzJosé M Mato

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.