Lipoarabinomannan of Mycobacterium tuberculosis promotes protein tyrosine dephosphorylation and inhibition of mitogen-activated protein kinase in human mononuclear phagocytes. Role of the Src homology 2 containing tyrosine phosphatase 1.

The Journal of Biological Chemistry
K L KnutsonN E Reiner

Abstract

Lipoarabinomannan (LAM) is a putative virulence factor of Mycobacterium tuberculosis that inhibits monocyte functions, and this may involve antagonism of cell signaling pathways. The effects of LAM on protein tyrosine phosphorylation in cells of the human monocytic cell line THP-1 were examined. LAM promoted tyrosine dephosphorylation of multiple cell proteins and attenuated phorbol 12-myristate 13-acetate-induced activation of mitogen-activated protein kinase. To examine whether these effects of LAM could be related to activation of a phosphatase, fractions from LAM-treated cells were analyzed for dephosphorylation of para-nitrophenol phosphate. The data show that LAM induced increased phosphatase activity associated with the membrane fraction. The Src homology 2 containing tyrosine phosphatase 1 (SHP-1) is important for signal termination and was examined as a potential target of LAM. Exposure of cells to LAM brought about (i) an increase in tyrosine phosphorylation of SHP-1, and (ii) translocation of the phosphatase to the membrane. Phosphatase assay of SHP-1 immunoprecipitated from LAM-treated cells, using phosphorylated mitogen-activated protein kinase as substrate, indicated that LAM promoted increased activity of SHP-1 i...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A RossomandoT W Sturgill
Jun 1, 1992·Trends in Biochemical Sciences·S L Pelech, J S Sanghera
Aug 21, 1992·Science·B R Bloom, C J Murray
Sep 23, 1991·FEBS Letters·P D Adams, P J Parker
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S L WeinsteinA L DeFranco
Apr 1, 1990·Clinical and Experimental Immunology·L D SibleyJ L Krahenbuhl
Jul 18, 1986·Science·Y Nishizuka
Aug 1, 1980·International Journal of Cancer. Journal International Du Cancer·S TsuchiyaK Tada
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Z ZhaoE H Fischer
Jun 23, 1995·The Journal of Biological Chemistry·M H Cobb, E J Goldsmith
Jan 27, 1995·Cell·G B CohenD Baltimore
Apr 1, 1993·Trends in Microbiology·S H Kaufmann, J D van Embden
Aug 1, 1994·Infection and Immunity·J GerckenH D Flad
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·R J MazzaccaroB R Bloom

❮ Previous
Next ❯

Citations

Jun 22, 1999·Immunologic Research·K M Coggeshall
Dec 29, 2004·Pathologie-biologie·Jean-Louis Herrmann, Philippe-Henri Lagrange
May 27, 2003·Biochimie·Jérôme NigouGermain Puzo
Feb 6, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·J S FriedlandJ-M Dayer
Jun 26, 2003·Trends in Microbiology·Ludovic TailleuxOlivier Neyrolles
Dec 28, 1999·Microbes and Infection·G R Strohmeier, M J Fenton
Apr 15, 2004·Nature Reviews. Microbiology·Anil KoulAxel Ullrich
Aug 18, 2000·Immunology and Cell Biology·E R Rhoades, H J Ullrich
Aug 28, 2010·Journal of Biochemistry·Hiroyuki MoriiYosuke Koga
Jan 10, 2002·Critical Care Medicine·Michael A West, Wyrta Heagy
Jun 3, 2009·Transboundary and Emerging Diseases·L Guenin-MacéC Demangel
Jul 19, 2000·Infection and Immunity·C Astarie-DequekerI Maridonneau-Parini
Sep 23, 2003·Infection and Immunity·Anne Lise K HestvikYossef Av-Gay
Apr 22, 2003·Infection and Immunity·Savita PrabhakarRichard Pine
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Isabelle VergneVojo Deretic
May 29, 2008·Future Microbiology·Renan Goude, Tanya Parish
May 20, 2005·American Journal of Veterinary Research·Douglas J WeissMitchell S Abrahamsen
Jan 17, 2014·Postgraduate Medical Journal·Pronoti SarkarBarnali Kakati
Mar 5, 2003·Cellular Microbiology·Jeffrey S Schorey, Andrea M Cooper
Jan 30, 2015·Frontiers in Cellular and Infection Microbiology·Isabelle VergneJérôme Nigou
Oct 24, 2014·Frontiers in Immunology·Somdeb BoseDasgupta, Jean Pieters
Dec 3, 2005·PLoS Pathogens·Graham R StewartDouglas B Young
Jan 15, 2011·Developmental and Comparative Immunology·Stephanie WiddisonTracey J Coffey
Jun 19, 2004·Bioorganic & Medicinal Chemistry Letters·K N JayaprakashBert Fraser-Reid
Aug 2, 2005·Biochemical and Biophysical Research Communications·Renata M S PereiraUlisses G Lopes
Aug 22, 2006·Immunobiology·Philipp Mueller, Jean Pieters
Feb 22, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Devki Nandan, Neil E Reiner

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.